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| Content Provider | Springer Nature Link |
|---|---|
| Author | Karaöz, Erdal Doğan, Burcu Nur Aksoy, Ayça Gacar, Gülçin Akyüz, Serap Ayhan, Selda Genç, Zehra Seda Yürüker, Sinan Duruksu, Gökhan Demircan, Pınar Çetinalp Sarıboyacı, Ayla Eker |
| Copyright Year | 2009 |
| Abstract | Dental pulp stem cells were primarily derived from the pulp tissues of exfoliated deciduous teeth, primary incisors and permanent third molar teeth. The aim of this study was to isolate and extensively characterise SCs derived from human natal dental pulp (hNDP). For characterisation, proliferation capacity, phenotypic properties, ultrastructural and differentiation characteristics and gene expression profiles were utilised. A comparison was done between the properties of NDP-SCs and the properties of mesenchymal stem cells (MSCs) from bone marrow (BM) of the human. Stem cells isolated from hNDP and hBM were analysed by flow cytometry, reverse transcriptase-PCR, Real Time-PCR, and immunocytochemistry. Both cell lines were directionally differentiated towards adipogenic, osteogenic chondrogenic, myogenic and neurogenic lineages. hNDP-SCs and hBM-MSCs expressed CD13, CD44, CD90, CD146 and CD166, but not CD3, CD8, CD11b, CD14, CD15, CD19, CD33, CD34, CD45, CD117, and HLA-DR. Ultrastructural characteristics of hNDP-SCs showed more developed and metabolically active cells. hNDP-SCs and hBM-MSCs expressed some adipogenic (leptin, adipophilin and PPARγ), myogenic (desmin, myogenin, myosinIIa, and α-SMA), neurogenic (γ-enolase, MAP2a,b, c-fos, nestin, NF-H, NF-L, GFAP and betaIII tubulin), osteogenic (osteonectin, osteocalcin, osteopontin, Runx-2, and type I collagen) and chondrogenic (type II collagen, SOX9) markers without any stimulation towards differentiation under basal conditions. Embryonic stem cell markers Oct4, Rex-1, FoxD-3, Sox2, and Nanog were also identified. The differentiation potential of hNDP-SCs and hBM-MSCs to adipogenic, osteogenic, chondrogenic, myogenic and neurogenic was shown. This report described the first successful isolation and characterisation of hNDP-SCs. |
| Starting Page | 95 |
| Ending Page | 112 |
| Page Count | 18 |
| File Format | |
| ISSN | 09486143 |
| Journal | Histochemistry and Cell Biology |
| Volume Number | 133 |
| Issue Number | 1 |
| e-ISSN | 1432119X |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2009-10-09 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Human Dental pulp Natal teeth Bone marrow Mesenchymal stem cell In vitro Characterisation Medicine/Public Health Anatomy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Histology Molecular Biology Medical Laboratory Technology |
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