NDLI: Visualization of the MCM DNA helicase at replication factories before the onset of DNA synthesis

Content Provider	Springer Nature Link
Author	Aparicio, Tomás Megías, Diego Méndez, Juan
Copyright Year	2012
Abstract	In mammalian cells, DNA synthesis takes place at defined nuclear structures termed “replication foci” (RF) that follow the same order of activation in each cell cycle. Intriguingly, immunofluorescence studies have failed to visualize the DNA helicase minichromosome maintenance (MCM) at RF, raising doubts about its physical presence at the sites of DNA synthesis. We have revisited this paradox by pulse-labeling RF during the S phase and analyzing the localization of MCM at labeled DNA in the following cell cycle. Using high-throughput confocal microscopy, we provide direct evidence that MCM proteins concentrate in G1 at the chromosome structures bound to become RF in the S phase. Upon initiation of DNA synthesis, an active “MCM eviction” mechanism contributes to reduce the excess of DNA helicases at RF. Most MCM complexes are released from chromatin, except for a small but detectable fraction that remains at the forks during the S phase, as expected for a replicative helicase.
Starting Page	499
Ending Page	507
Page Count	9
File Format	PDF
ISSN	00095915
Journal	Chromosoma
Volume Number	121
Issue Number	5
e-ISSN	14320886
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-08-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Eukaryotic Microbiology Animal Genetics and Genomics Human Genetics Cell Biology Biochemistry Developmental Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Genetics (clinical)

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