NDLI: Microinjection of antibodies to centromere protein CENP-A arrests cells in interphase but does not prevent mitosis

Content Provider	Springer Nature Link
Author	Figueroa, Javier Saffrich, Rainer Ansorge, Wilhelm Valdivia, Manuel
Copyright Year	1998
Abstract	Centromere protein CENP-A is a histone H3-like protein associated specifically with the centromere and represents one of the human autoantigens identified by sera taken from patients with the CREST variant of progressive systemic sclerosis. Injection of whole human autoimmune serum to the centromere into interphase cells disrupts some mitotic events. It has been assumed that this effect is due to CENP-E and CENP-C autoantigens, because of the effects of injecting monospecific sera to those proteins into culture cells. Here we have used an antibody raised against an N-terminal peptide of the human autoantigen CENP-A to determine its function in mitosis and during cell cycle progression. Affinity-purified anti-CENP-A antibodies injected into the nucleus during the early replication stages of the cell cycle caused cells to arrest in interphase before mitosis. These cells showed highly condensed small nuclei, a granular cytoplasm and loss of their division capability. On the other hand, microinjection of nocodazole-blocked HeLa cells in mitosis resulted in the typical punctate staining pattern of CENP-A for centromeres during different stages of mitosis and apparently normal cell division. This was corroborated by time-lapse imaging microscopy analysis of mid-interphase-injected cells, revealing that they undergo mitosis and divide properly. However, a significant delay throughout the progression of mitotic stages was observed. These results suggest that CENP-A is involved predominantly in an essential interphase event at the centromere before mitosis. This may include chromatin assembly at the kinetochore coordinate with late replication of satellite DNA to form an active centromere.
Starting Page	397
Ending Page	405
Page Count	9
File Format	PDF
ISSN	00095915
Journal	Chromosoma
Volume Number	107
Issue Number	6-7
e-ISSN	14320886
Language	English
Publisher	Springer-Verlag
Publisher Date	1998-12-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Genetics (clinical)

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Microinjection of antibodies to centromere protein CENP-A arrests cells in interphase but does not prevent mitosis

Distinct modes of centromere protein dynamics during cell cycle progression in Drosophila S2R+ cells

CENP-B: a major human centromere protein located beneath the kinetochore

Evolutionary insights into the role of the essential centromere protein CAL1 in Drosophila

Evolutionary insights into the role of the essential centromere protein CAL1 in Drosophila

CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cells

Phosphorylation of CENP-A on serine 7 does not control centromere function

Anti-centromere antibodies in a large cohort of systemic sclerosis patients: comparison between immunofluorescence, CENP-A and CENP-B ELISA.

Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant

Microinjection of antibodies to centromere protein CENP-A arrests cells in interphase but does not prevent mitosis

Similar Documents

Microinjection of antibodies to centromere protein CENP-A arrests cells in interphase but does not prevent mitosis

Distinct modes of centromere protein dynamics during cell cycle progression in Drosophila S2R+ cells

CENP-B: a major human centromere protein located beneath the kinetochore

Evolutionary insights into the role of the essential centromere protein CAL1 in Drosophila

Evolutionary insights into the role of the essential centromere protein CAL1 in Drosophila

CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cells

Phosphorylation of CENP-A on serine 7 does not control centromere function

Anti-centromere antibodies in a large cohort of systemic sclerosis patients: comparison between immunofluorescence, CENP-A and CENP-B ELISA.

Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant

Microinjection of antibodies to centromere protein CENP-A arrests cells in interphase but does not prevent mitosis