NDLI: Fenugreek increases insulin-stimulated creatine content in L6C11 muscle myotubes

Content Provider	Springer Nature Link
Author	Tomcik, Kristyen A. Smiles, William J. Camera, Donny M. Hügel, Helmut M. Hawley, John A. Watts, Rani
Copyright Year	2016
Abstract	Creatine uptake by muscle cells is increased in the presence of insulin. Accordingly, compounds with insulin-like actions may also augment creatine uptake. The aim of this study was to investigate whether Trigonella foenum-graecum (fenugreek), an insulin mimetic, increases total intracellular creatine levels in vitro.Total cellular creatine content was measured fluorometrically in L6C11 muscle myotubes treated for 1, 4, and 24 h with 0.5 mM creatine (CR), CR and 20 μg/mL fenugreek seed extract (CR + FEN), CR and 100 nM insulin (CR + INS), and CR + INS + FEN (n = 6 per treatment group). Alterations in the expression of the sodium- and chloride-dependent creatine transporter, SLC6A8, and key signaling proteins in the PI3-K/Akt pathway were determined.Compared to control (CON), CR + INS + FEN increased total creatine content after 4 h (P < 0.05), whereas all conditions increased SLC6A8 protein expression above CON at this time (P < 0.05). Changes in insulin signaling were demonstrated via increases in AktThr308 phosphorylation, with CR + INS > CON and CR at 1 h (P < 0.05) and with CR + INS + FEN > CON, CR, and CR + INS at 4 h (P < 0.05). In contrast, no changes in PKCζ/λ or GLUT4 phosphorylation were detected.Fenugreek, when combined with insulin, modulates creatine content via a mechanism which is independent of the activity of SLC6A8, suggesting that an alternative mechanism is responsible for the regulation and facilitation of insulin-mediated creatine uptake in skeletal muscle cells.
Starting Page	973
Ending Page	979
Page Count	7
File Format	PDF
ISSN	14366207
Journal	European Journal of Nutrition
Volume Number	56
Issue Number	3
e-ISSN	14366215
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-01-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Creatine content Creatine transport Fenugreek supplement Insulin signaling Nutrition
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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