NDLI: Suitability of a batch in vitro fermentation model using human faecal microbiota for prediction of conversion of flaxseed lignans to enterolactone with reference to an in vivo rat model

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Suitability of a batch in vitro fermentation model using human faecal microbiota for prediction of conversion of flaxseed lignans to enterolactone with reference to an in vivo rat model

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Suitability of a batch in vitro fermentation model using human faecal microbiota for prediction of conversion of flaxseed lignans to enterolactone with reference to an in vivo rat model

Content Provider	Springer Nature Link
Author	Aura, A. M. Oikarinen, S. Mutanen, M. Heinen, S. M. Adlercreutz, H. C. T. Virtanen, H. Poutanen, K. S.
Copyright Year	2005
Abstract	In vitro fermentation models have been used widely for studies of shortchain fatty acid (SCFA) formation from carbohydrates, whereas the suitability of these methods for enterolactone (ENL) formation has received less attention. The aim was to study the suitability of an in vitro fermentation model for prediction of bioconversion of lignans to ENL, to compare the approach with that of an in vivo rat model and to study the SCFA formation in both models.Predigested samples of rye bran (R), flaxseed meal (F) alone, or in combination with rye bran (R&F) and a faecal control were incubated in an in vitro fermentation model using human faecal microbiota. In the in vivo experiment rats consumed a non–fibre control diet (C) or diets supplemented either with rye bran (R), flaxseed meal (F) alone, or with their combination (R&F) for four weeks. Enterodiol (END), ENL and SCFA concentrations were measured from in vitro faecal fermentation samples and from the intestinal contents of rats. Plasma ENL concentrations from rats were also measured.The highest ENL production was found in vitro with the F supplement (areas under curve: 740 ± 4, 7500 ± 400, 2600 ± 500 and 1520 ± 70 nmol · h for the R, F, R&F supplements and faecal control, respectively). In vivo, the concentration of ENL in caecal digesta from flaxseed meal was significantly (P < 0.05) enhanced by the presence of rye bran (medians 261, 407 and 24 nmol/g in the F, R&F and C groups, respectively). No correlation was found between the models regarding ENL production, possibly due to different responses to the presence of rye bran matrix, differences in microbiota or application of a batch in the in vitro fermentation model. Rye bran supplementation enhanced butyrate production both in vitro and in vivo.In vitro fermentation and the in vivo rat models responded differently to the presence of rye bran and no correlation with regard to the ENL formation from flaxseed lignans was observed.
Starting Page	45
Ending Page	51
Page Count	7
File Format	PDF
ISSN	14366207
Journal	European Journal of Nutrition
Volume Number	45
Issue Number	1
e-ISSN	14366215
Language	English
Publisher	Steinkopff-Verlag
Publisher Date	2005-04-25
Publisher Place	Darmstadt
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	lignans nondigestible carbohydrates in vitro faecal fermentation in vivo rat model Nutrition
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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