NDLI: Metabolic compartmentation in rainbow trout cardiomyocytes: coupling of hexokinase but not creatine kinase to mitochondrial respiration

Content Provider	Springer Nature Link
Author	Karro, Niina Sepp, Mervi Jugai, Svetlana Laasmaa, Martin Vendelin, Marko Birkedal, Rikke
Copyright Year	2016
Abstract	Rainbow trout (Oncorhynchus mykiss) cardiomyocytes have a simple morphology with fewer membrane structures such as sarcoplasmic reticulum and t-tubules penetrating the cytosol. Despite this, intracellular ADP diffusion is restricted. Intriguingly, although diffusion is restricted, trout cardiomyocytes seem to lack the coupling between mitochondrial creatine kinase (CK) and respiration. Our aim was to study the distribution of diffusion restrictions in permeabilized trout cardiomyocytes and verify the role of CK. We found a high activity of hexokinase (HK), which led us to reassess the situation in trout cardiomyocytes. We show that diffusion restrictions are more prominent than previously thought. In the presence of a competitive ADP-trapping system, ADP produced by HK, but not CK, was channeled to the mitochondria. In agreement with this, we found no positively charged mitochondrial CK in trout heart homogenate. The results were best fit by a simple mathematical model suggesting that trout cardiomyocytes lack a functional coupling between ATPases and pyruvate kinase. The model simulations show that diffusion is restricted to almost the same extent in the cytosol and by the outer mitochondrial membrane. Furthermore, they confirm that HK, but not CK, is functionally coupled to respiration. In perspective, our results suggest that across a range of species, cardiomyocyte morphology and metabolism go hand in hand with cardiac performance, which is adapted to the circumstances. Mitochondrial CK is coupled to respiration in adult mammalian hearts, which are specialized to high, sustained performance. HK associates with mitochondria in hearts of trout and neonatal mammals, which are more hypoxia-tolerant.
Starting Page	103
Ending Page	116
Page Count	14
File Format	PDF
ISSN	01741578
Journal	Journal of Comparative Physiology B
Volume Number	187
Issue Number	1
e-ISSN	1432136X
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-08-13
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ADP diffusion restriction Creatine kinase Heart Hexokinase Metabolic compartmentation Rainbow trout Animal Physiology Biomedicine Human Physiology Zoology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Physiology Biochemistry Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Endocrinology

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