NDLI: Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica

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Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica

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Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica

Content Provider	Springer Nature Link
Author	Shen, Jihong Kan, Guangfeng Shi, Cuijuan Lei, Zhenhuan Xie, Qiuju Qian, Wenjia
Copyright Year	2011
Abstract	A bacterium hydrolyzing carboxymethylcellulose, isolated from Antarctic sea ice, was identified as Pseudoalteromonas sp. based on 16S rDNA gene sequences and named as Pseudoalteromonas sp. AN545. The extracellular endo-1,4-β-glucanase AN-1 was purified successively by ammonium sulfate precipitation, DEAE-Sepharose ion exchange chromatography and Sephadex G-75 gel filtration chromatography. The molecular mass of AN-1 was estimated to be 47.5 kDa utilizing SDS-PAGE and gel chromatography analysis. AN-1 could hydrolyze caboxymethylcellulose, avicel and β-glucan, but not cellobiose, xylan and p-Nitrophenyl-β-D-glucopyranoside. The optimal temperature and pH for the β-glucanase activity of AN-1 were determined to be at 30°C and pH 6.0, respectively. AN-1 was stable at acidic solutions of pH 5.0–6.5 and temperatures below 30°C for 1 h. Moreover, the specific activity was enhanced by Ca2+ and Mg2+, and inhibited by Cu2+. The kinetic parameters Michaelis constant (K m) and maximum velocity (V max) of AN-1 were 3.96 mg/mL and 6.06×10−2 mg/(min·mL), respectively.
Starting Page	1086
Ending Page	1092
Page Count	7
File Format	PDF
ISSN	02544059
Journal	Chinese Journal of Oceanology and Limnology
Volume Number	29
Issue Number	5
e-ISSN	19935005
Language	English
Publisher	SP Science Press
Publisher Date	2011-08-08
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	Pseudoalteromonas endo-1,4-β-glucanase cold-active enzyme Antarctic sea ice stability Oceanography
Content Type	Text
Resource Type	Article
Subject	Water Science and Technology Oceanography

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Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica