NDLI: Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

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Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection

Content Provider	Springer Nature Link
Author	Luo, Zhan Zhang, Jiquan Li, Fuhua Zhang, Xiaojun Liu, Chengzhang Xiang, Jianhai
Copyright Year	2011
Abstract	Acting as one of the pattern recognition receptors (PRRs), C-type lectin is believed to mediate pathogen recognition and plays an important role in the clearance of pathogens as part of the innate immune system. In this work, a novel C-type lectin gene (named LvLec1) was cloned from the shrimp Litopenaeus vannamei. The ORF of LvLec1 is 510 bp, encoding 169 amino acids. The deduced amino acid sequence contains a putative signal peptide of 19 amino acids at the N-terminal and a carbohydrate recognition domain (CRD) at the C-terminal. LvLec1 was mainly expressed in the hepatopancreas. Real-time PCR analysis indicated that the level of LvLec1 transcripts significantly changed in the hepatopancreas after the shrimp were artificially challenged with LPS, Micrococcus lysodeikticus and white spot syndrome virus (WSSV). RNAi-based silencing of LvLec1 resulted in increases in mortality when the shrimp were challenged with WSSV, and the median lethal time was reduced compared with controls. Although there was no characteristic “EPN” (Glu-Pro-Ser) or “QPD” (Gln-Pro-Asp) motif, the recombinant LvLec1, expressed in Escherichia coli BL21 (DE3), could also agglutinate M. lysodeikticus and Vibrio anguillarum. The agglutinating activities were calcium-dependent and could be inhibited by D-mannose, D-glucose, D-galactose and N-Acetyl-D-mannose. These results suggest that LvLec1 might be involved in the immune response against WSSV and bacterial infections and contribute to non-self recognition as a pattern recognition receptor in the innate immune system of the shrimp L. vannamei.
Starting Page	942
Ending Page	951
Page Count	10
File Format	PDF
ISSN	02544059
Journal	Chinese Journal of Oceanology and Limnology
Volume Number	29
Issue Number	5
e-ISSN	19935005
Language	English
Publisher	SP Science Press
Publisher Date	2011-08-08
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	Litopenaeus vannamei innate immune pattern recognition receptor C-type lectin RNA interference (RNAi) recombinant expression Oceanography
Content Type	Text
Resource Type	Article
Subject	Water Science and Technology Oceanography

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Identification of a novel C-type lectin from the shrimp Litopenaeus vannamei and its role in defense against pathogens infection