NDLI: Genetical and physical assignments of equine microsatellites—first integration of anchored markers in horse genome mapping

Content Provider	Springer Nature Link
Author	Breen, Matthew Lindgren, Gabriella Binns, Matthew M. rman, Julianne Irvin, Zlaka Bell, Kevin Sandberg, Kaj Ellegren, Hans
Copyright Year	1997
Abstract	Twenty equine microsatellites were isolated from a ge-nomic phage library, and their genetical and physical localization was sought by linkage mapping and fluorescent in situ hybridization (FISH). Nineteen of the markers were found to be polymorphic with, in most cases, heterozygosities exceeding 50%. The markers were mapped in a Swedish reference family for gene mapping, comprising eight half-sib families from Standardbred and Icelandic horse sires. Segregation was analyzed against a set of 35 other markers typed in the pedigree. Thirteen of the microsatellites showed linkage to at least one other marker, with a total of 21 markers being involved in these linkages. In parallel, 18 of the microsatellites could be assigned to their chromosomal region by FISH. These assignments involved eight equine autosomes: ECA1, 2, 4, 6, 9, 10, 15, and 16. The genetical and physical mappings revealed by this study represent a significant extension of the current knowledge of the equine genome map.
Starting Page	267
Ending Page	273
Page Count	7
File Format	PDF
ISSN	09388990
Journal	Mammalian Genome
Volume Number	8
Issue Number	4
e-ISSN	14321777
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell Biology Anatomy Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics

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