NDLI: A yeast artificial chromosome (YAC) library containing 10 haploid chicken genome equivalents

Content Provider	Springer Nature Link
Author	Toye, Ayo A. Schalkwyk, Leonard Lehrach, Hans Bumstead, Nat
Copyright Year	1997
Abstract	We report the construction of a YAC library that provides 10-fold redundant coverage of the chicken genome. The library was made by transforming S. cerevisiae AB1380 with YAC constructs consisting of partially digested and size fractionated (>465 kb) EcoRI genomic fragments ligated to pCGS966 YAC vector arms. The primary library provides 8.5-fold redundant coverage and consists of 16,000 clones arrayed in duplicate 96-well microtiter plates and gridded on nylon membranes at high density (18,000 clones/484cm2). The average insert size, 634 kb, was derived from size fractionation of a random sample of 218 YACs. Hybridization of five unlinked chicken genes to colony blots revealed six or more positive clones. This is consistent with the theoretical expectation from average insert sizes and number of clones. A second collection of clones consists of a further 20,000 colonies, of which 20% contain inserts larger than 450 kb and 80% contain only coligated vector arms. We estimate that these clones provide a further 1.5-fold redundant coverage of the chicken genome; thus, the total collection of 36,000 clones provides 10-fold redundant coverage of the chicken genome. The library is intended as a resource for fine-scale analysis of the organization of the chicken genome and is presently being used to construct a contig map of chicken Chromosome (Chr) 16, which contains the MHC and nucleolar organizer.
Starting Page	274
Ending Page	276
Page Count	3
File Format	PDF
ISSN	09388990
Journal	Mammalian Genome
Volume Number	8
Issue Number	4
e-ISSN	14321777
Language	English
Publisher	Springer-Verlag
Publisher Date	1997-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell Biology Anatomy Zoology
Content Type	Text
Resource Type	Article
Subject	Genetics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

A bovine YAC library containing four- to five-fold genome equivalents

Construction of a large-insert yeast artificial chromosome library of the rat genome

Construction of a large-insert yeast artificial chromosome library of the rat genome

Construction and characterization of a new bovine bacterial artificial chromosome library with 10 genome-equivalent coverage

Construction and characterization of a porcine P1-derived artificial chromosome (PAC) library covering 3.2 genome equivalents and cytogenetical assignment of six type I and type II loci

Construction and characterization of a sheep BAC library of three genome equivalents

Construction and extensive characterization of a goat Bacterial Artificial Chromosome library with threefold genome coverage

Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

A yeast artificial chromosome (YAC) library containing 10 haploid chicken genome equivalents

Similar Documents

Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

A bovine YAC library containing four- to five-fold genome equivalents

Construction of a large-insert yeast artificial chromosome library of the rat genome

Construction of a large-insert yeast artificial chromosome library of the rat genome

Construction and characterization of a new bovine bacterial artificial chromosome library with 10 genome-equivalent coverage

Construction and characterization of a porcine P1-derived artificial chromosome (PAC) library covering 3.2 genome equivalents and cytogenetical assignment of six type I and type II loci

Construction and characterization of a sheep BAC library of three genome equivalents

Construction and extensive characterization of a goat Bacterial Artificial Chromosome library with threefold genome coverage

Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.

A yeast artificial chromosome (YAC) library containing 10 haploid chicken genome equivalents