NDLI: A cotton fiber-preferential promoter, PGbEXPA2, is regulated by GA and ABA in Arabidopsis

Content Provider	Springer Nature Link
Author	Li, Yang Tu, Lili Ye, Zhengxiu Wang, Maojun Gao, Wenhui Zhang, Xianlong
Copyright Year	2015
Abstract	PGbEXPA2 (Promoter of GbEXPA2 ) was preferentially and strongly expressed during cotton fiber development, and the 461-bp PGbEXPA2 fragment was essential for responding to exogenous GA and ABA in Arabidopsis.Cotton fibers are highly elongated single-cell, unbranched and non-glandular seed trichomes. Previous studies have reported that the transcript level of GbEXPA2 is significantly up-regulated during fiber cell elongation, suggesting that GbEXPA2 has an important function in fiber development. In this study, the promoter of GbEXPA2 (839 bp) from the DT sub-genome was isolated from Gossypium barbadense 3–79. Consistent with the expression pattern of GbEXPA2, the promoter PGbEXPA2 was able to express GUS to high levels in elongating fibers, but not in the root, stem, or leaf. In Arabidopsis, GUS activity was only found in the rosette leaf trichomes and rosette leaf vascular tissue, indicating that the transcription factors which bind to PGbEXPA2 in the leaf trichomes of transgenic Arabidopsis were similar to those found in cotton fiber. A deletion analysis of PGbEXPA2 revealed that a 461-bp fragment was sufficient to drive GUS expression in cotton fibers and Arabidopsis rosette leaf trichomes. Exogenous phytohormonal treatments on transgenic Arabidopsis with different promoter lengths (P-839, P-705, P-588 and P-461) showed that GUS activity in Arabidopsis trichomes could be strongly up-regulated by GA and, in contrast, down-regulated by ABA.
Starting Page	1539
Ending Page	1549
Page Count	11
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	34
Issue Number	9
e-ISSN	1432203X
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-05-22
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cotton fiber α-expansin Promoter analysis Gibberellins Abscisic acid Plant Sciences Cell Biology Biotechnology Plant Biochemistry
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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