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| Content Provider | Springer Nature Link |
|---|---|
| Author | Chun, Ju Hyeon Adhikari, Prakash Babu Park, Seong Bum Han, Jung Yeon Choi, Yong Eui |
| Copyright Year | 2015 |
| Abstract | Protopanaxadiol (PPD) is an aglycone of dammarene-type ginsenoside and has high medicinal values. In this work, we reported the PPD production in transgenic tobacco co-overexpressing PgDDS and CYP716A47.PPD is an aglycone of ginsenosides produced by Panax species and has a wide range of pharmacological activities. PPD is synthesized via the hydroxylation of dammarenediol-II (DD) by CYP716A47 enzyme. Here, we established a PPD production system via cell suspension culture of transgenic tobacco co-overexpressing the genes for PgDDS and CYP716A47. The concentration of PPD in transgenic tobacco leaves was 2.3–5.7 µg/g dry weight (DW), depending on the transgenic line. Leaf segments were cultured on medium with various types of hormones to induce callus. Auxin treatment, particularly 2,4-D, strongly enhanced the production of DD (783.8 µg g−1 DW) and PPD (125.9 µg g−1 DW). Treatment with 2,4-D enhanced the transcription of the HMG-Co reductase (HMGR) and squalene epoxidase genes. PPD production reached 166.9 and 980.9 µg g−1 DW in a 250-ml shake flask culture and in 5-l airlift bioreactor culture, respectively. |
| Starting Page | 1551 |
| Ending Page | 1560 |
| Page Count | 10 |
| File Format | |
| ISSN | 07217714 |
| Journal | Plant Cell Reports |
| Volume Number | 34 |
| Issue Number | 9 |
| e-ISSN | 1432203X |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2015-05-17 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | CYP716A47 Dammarenediol-II synthase Protopanaxadiol Sapogenin Transgenic tobacco Plant Sciences Cell Biology Biotechnology Plant Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Medicine Agronomy and Crop Science |
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