NDLI: Efficient transformation of Kalanchoe blossfeldiana and production of male-sterile plants by engineered anther ablation

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Efficient transformation of Kalanchoe blossfeldiana and production of male-sterile plants by engineered anther ablation

Content Provider	Springer Nature Link
Author	García Sogo, Begoña Pineda, Benito Castelblanque, Lourdes Antón, Teresa Medina, Mónica Roque, Edelín Torresi, Claudia Beltrán, José Pío More, Vicente Cañas, Luis Antonio
Copyright Year	2009
Abstract	Engineered male sterility in ornamental plants has many applications such as facilitate hybrid seed production, eliminate pollen allergens, reduce the need for deadheading to extend the flowering period, redirect resources from seeds to vegetative growth, increase flower longevity and prevent gene flow between genetically modified and related native plants. We have developed a reliable and efficient Agrobacterium-mediated protocol for the genetic transformation of different Kalanchoe blossfeldiana commercial cultivars. Transformation efficiency for cv. ‘Hillary’ was 55.3% whereas that of cv. ‘Tenorio’ reached 75.8%. Selection was carried out with the nptII gene and increasing the kanamycin concentration from 25 to 100 mg l−1 allowed to reduced escapes from 50 to 60% to virtually 0%. This method was used to produce male-sterile plants through engineered anther ablation. In our approach, we tested a male sterility chimaeric gene construct (PsEND1::barnase) to evaluate its effectiveness and effect on phenotype. No significant differences were found in the growth patterns between the transgenic lines and the wild-type plants. No viable pollen grains were observed in the ablated anthers of any of the lines carrying the PsEND1::barnase construct, indicating that the male sterility was complete. In addition, seed set was completely abolished in all the transgenic plants obtained. Our engineered male-sterile approach could be used, alone or in combination with a female-sterility system, to reduce the invasive potential of new ornamentals, which has become an important environmental problem in many countries.
Starting Page	61
Ending Page	77
Page Count	17
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	29
Issue Number	1
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-11-17
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Kalanchoe blossfeldiana Engineered male sterility PsEND1 promoter Barnase Anther ablation Biosafe ornamentals Plant Biochemistry Biotechnology Plant Sciences Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

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Efficient transformation of Kalanchoe blossfeldiana and production of male-sterile plants by engineered anther ablation