NDLI: Expression of a glycosylated GFP as a bivalent reporter in exocytosis

Please wait, while we are loading the content...

In vitro conservation of oil palm somatic embryos for 20 years on a hormone-free culture medium: characteristics of the embryogenic cultures, derived plantlets and adult palms

Isolation and promoter analysis of anther-specific genes encoding putative arabinogalactan proteins in Malus × domestica

Vitamins C and E improve regrowth and reduce lipid peroxidation of blackberry shoot tips following cryopreservation

Transcriptional differences in gene families of the ascorbate–glutathione cycle in wheat during mild water deficit

Tissue culture-induced variation at simple sequence repeats in sorghum (Sorghum bicolor L.) is genotype-dependent and associated with down-regulated expression of a mismatch repair gene, MLH3

Efficient transformation of Kalanchoe blossfeldiana and production of male-sterile plants by engineered anther ablation

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

Manipulation of amino acid composition in soybean seeds by the combination of deregulated tryptophan biosynthesis and storage protein deficiency

Pre- and post-agroinfection strategies for efficient leaf disk transformation and regeneration of transgenic strawberry plants

Erratum to: Green-tissue-specific, C4-PEPC-promoter-driven expression of Cry1Ab makes transgenic potato plants resistant to tuber moth (Phthorimaea operculella, Zeller)

Plant Cell Reports : Volume 28

Plant Cell Reports : Volume 27

Plant Cell Reports : Volume 26

Plant Cell Reports : Volume 25

Plant Cell Reports : Volume 24

Plant Cell Reports : Volume 23

Plant Cell Reports : Volume 22

Plant Cell Reports : Volume 21

Plant Cell Reports : Volume 20

Plant Cell Reports : Volume 19

Plant Cell Reports : Volume 18

Plant Cell Reports : Volume 17

Plant Cell Reports : Volume 16

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

Content Provider	Springer Nature Link
Author	Paris, Nadine Saint Jean, Bru Faraco, Marianna Krzeszowiec, Weronika Dalessandro, Giuseppe Neuhaus, Jean Marc Di Sansebastia, Gian Pietro
Copyright Year	2009
Abstract	The complex-type N-linked glycans of plants differ markedly in structure from those of animals. Like those of insects and mollusks they lack terminal sialic acid(s) and may contain an α-(1,3)-fucose (Fuc) linked to the proximal GlcNAc residue and/or a β-(1,2)-xylose (Xyl) residue attached to the proximal mannose (Man) of the glycan core. N-glycosylated GFPs were used in previous studies showing their effective use to report on membrane traffic between the ER and the Golgi apparatus in plant cells. In all these cases glycosylated tags were added at the GFP termini. Because of the position of the tag and depending on the sorting and accumulation site of these modified GFP, there is always a risk of processing and degradation, and this protein design cannot be considered ideal. Here, we describe the development of three different GFPs in which the glycosylation site is internally localized at positions 80, 133, or 172 in the internal sequence. The best glycosylation site was at position 133. This glycosylated GFPgl133 appears to be protected from undesired processing of the glycosylation site and represents a bivalent reporter for biochemical and microscopic studies. After experimental validation, we can conclude that amino acid 133 is an effective glycosylation site and that the GFPgl133 is a powerful tool for in vivo investigations in plant cell biology.
Starting Page	79
Ending Page	86
Page Count	8
File Format	PDF
ISSN	07217714
Journal	Plant Cell Reports
Volume Number	29
Issue Number	1
e-ISSN	1432203X
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-12-02
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	GFP Glycosylation Protoplast Secretion Plant Biochemistry Biotechnology Plant Sciences Cell Biology
Content Type	Text
Resource Type	Article
Subject	Plant Science Medicine Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Expression of a glycosylated GFP as a bivalent reporter in exocytosis.

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

A strong root-specific expression system for stable transgene expression in bread wheat

Protoplast isolation and transient gene expression in the single-cell C4 species, Bienertia sinuspersici

Estrogen-inducible GFP expression patterns in rice (Oryza sativa L.)

High expression of transgene protein in Spirodela

Genetic transformation of Arabidopsis lyrata: specific expression of the green fluorescent protein (GFP) in pistil tissues

A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean

Activity of the 5′ regulatory regions of the rice polyubiquitin rubi3 gene in transgenic rice plants as analyzed by both GUS and GFP reporter genes

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

Similar Documents

Expression of a glycosylated GFP as a bivalent reporter in exocytosis.

Expression of a glycosylated GFP as a bivalent reporter in exocytosis

A strong root-specific expression system for stable transgene expression in bread wheat

Protoplast isolation and transient gene expression in the single-cell C4 species, Bienertia sinuspersici

Estrogen-inducible GFP expression patterns in rice (Oryza sativa L.)

High expression of transgene protein in Spirodela

Genetic transformation of Arabidopsis lyrata: specific expression of the green fluorescent protein (GFP) in pistil tissues

A soybean (Glycine max) polyubiquitin promoter gives strong constitutive expression in transgenic soybean

Activity of the 5′ regulatory regions of the rice polyubiquitin rubi3 gene in transgenic rice plants as analyzed by both GUS and GFP reporter genes

Expression of a glycosylated GFP as a bivalent reporter in exocytosis