NDLI: Complementary mating types of Mucor circinelloides show electrophoretic karyotype heterogeneity

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Complementary mating types of Mucor circinelloides show electrophoretic karyotype heterogeneity

Content Provider	Springer Nature Link
Author	Díaz Mínguez, José María López Matas, M. Angeles Eslava, Arturo P.
Copyright Year	1999
Abstract	Electrophoretic karyotypes of ten strains of Mucor circinelloides f. lusitanicus were generated by contour-clamped homogeneous electric field (CHEF) gel-electrophoresis. Most of the strains analyzed showed polymorphisms, but a different main karyotype pattern could be correlated with each mating type. Genome structure was further analyzed by gene assignment to the chromosome-sized DNAs. Nonradioactive hybridization techniques identified the chromosomal localization of seven cloned genes. The hybridization patterns confirmed the similarity between the mating-type (−) strains and showed some heterogeneity among the mating-type (+) strains. Linkage was found between genes pyrF and chs3 in all the strains, between the gene leuA and the rDNA in all mating-type (−) strains and ATCC 1216b (+), and between chs2 and the rDNA in CBS 969.68 (+). This is the first time that gene linkage in M. circinelloides has been reported, but in some cases the linkage relationships obtained are strain-dependent.
Starting Page	383
Ending Page	389
Page Count	7
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	36
Issue Number	6
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-12-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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