NDLI: Use of sulfite resistance in Saccharomyces cerevisiae as a dominant selectable marker

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Use of sulfite resistance in Saccharomyces cerevisiae as a dominant selectable marker

Content Provider	Springer Nature Link
Author	Park, Hoon Lopez, Nathan I. Bakalinsky, Alan T.
Copyright Year	1999
Abstract	Two S. cerevisiae genes were found to exhibit dominant phenotypes useful for selecting transformants of industrial and laboratory strains of S. cerevisiae. FZF1-4, which confers sulfite resistance, was originally isolated and identified as RSU1-4, but the two genes are shown here to be allelic. Cysteine 57 in wild-type Fzf1p was found to be replaced by tyrosine in Fzf1-4p. Multicopy SSU1, which also confers sulfite resistance, was found to be somewhat less efficient. In both cases, a period of outgrowth in non-selective medium following transformation was found to be necessary. The number of transformants obtained was found to be strain-dependent, and also to depend on the sulfite concentration used during selection. Undesirable background growth of non-transformants was not observed at cell densities as high as 2.5 × 107/plate. In two ura3 laboratory strains where selection for URA3 was applied independently of that for sulfite, the transformation efficiency for sulfite resistance was about 50% that for uracil prototrophy.
Starting Page	339
Ending Page	344
Page Count	6
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	36
Issue Number	6
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-12-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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