NDLI: RPD3 (REC3) mutations affect mitotic recombination in Saccharomyces cerevisiae

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Current Genetics : Volume 35, Issue 1, February 1999

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Current Genetics : Volume 33

Current Genetics : Volume 32

Current Genetics : Volume 31

RPD3 (REC3) mutations affect mitotic recombination in Saccharomyces cerevisiae

Content Provider	Springer Nature Link
Author	Dora, E. G. Rudin, N. Martell, José R. Esposito, Michael S. Ramírez, R. M.
Copyright Year	1999
Abstract	Prior research identified the recessive rec3-1ts mutation in Saccharomyces cerevisiae which, in homozygous diploid cells, confers a conditional phenotype resulting in reduced levels of spontaneous mitotic recombination and loss of sporulation at the restrictive temperature of 36 °C. We found that a 3.4-kb genomic fragment that complements the rec3-1ts/rec3-1ts mutation and which maps to chromosome XIV, is identical to RPD3, a gene encoding a histone de-acetylase. Sporulation is reduced in homozygous diploid strains containing the rec3-1ts allele at 24 °C, suggesting that this allele of RPD3 encodes a gene product with a reduced function. Sporulation is abolished in diploid strains homozygous for the rpd3Δ or rec3-1ts alleles, as well as in rpd3Δ/rec3-1ts heteroallelic diploids, at the non-permissive temperature. Acid-phosphatase expression has been shown to be RPD3 dependent. We found that acid-phosphatase activity is greater in diploid strains homozygous for the temperature-sensitive rec3-1ts allele than in RPD3/RPD3 strains and increased further when mutant strains are grown at 36 °C. We also tested the rpd3Δ/rpd3Δ strains for their effects on spontaneous mitotic recombination. By assaying a variety of intra- and inter-genic recombination events distributed over three chromosomes, we found that in the majority of cases spontaneous mitotic recombination was reduced in diploid rpd3Δ/rpd3Δ cells (relative to a RPD3/RPD3 control). Finally, although 90% of mitotic recombinant events are initiated in the G1 phase of the growth cycle (i.e., before DNA synthesis) we show that RPD3 is not regulated in a cell-cycle-dependent manner. These data suggest that mitotic recombination, in addition to gene expression, is affected by changes in chromatin architecture mediated by RPD3.
Starting Page	68
Ending Page	76
Page Count	9
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	35
Issue Number	2
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-03-09
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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