NDLI: Studies of mitochondrial morphology and DNA amount in the rice egg cell

Content Provider	Springer Nature Link
Author	Takanashi, Hideki Ohnishi, Takayuki Mogi, Mirai Okamoto, Takashi Arimura, Shin ichi Tsutsumi, buhiro
Copyright Year	2009
Abstract	In plant vegetative cells, mitochondria are usually small and grain-shaped. In contrast, unusually shaped giant mitochondria (large cup-shaped or long stretched-rod-shaped) appear in the egg cells of geranium, maize, Ipomoea nil, and bracken. In this study, to characterize egg cell mitochondria in rice, we used nonenzymatic manual dissection to isolate unfertilized egg cells of rice and observed the egg cell mitochondria and mitochondrial DNA (mtDNA) simultaneously. These observations showed that the mitochondria in the rice egg cell are small and grain-shaped, unlike the mitochondria in geranium, maize, I. nil, and bracken. Double staining of mitochondria by MitoTracker and mtDNA by SYBR Green I showed that mitochondria in the rice egg cell have a large amount of mtDNA compared with the rice root protoplast. We also used real-time PCR analysis to quantify the mtDNA amount in the rice egg cell. We quantified the copy numbers of four mitochondrial genes per single rice egg cell and rice leaf protoplast. Real-time PCR analysis revealed that the egg cell has more than ten times more copy numbers of all of four genes encoded in the mitochondrial genome compared with the leaf protoplast.
Starting Page	33
Ending Page	41
Page Count	9
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	56
Issue Number	1
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-12-05
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rice Egg cell Mitochondria Mitochondrial DNA Proteomics Plant Sciences Cell Biology Biochemistry Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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