NDLI: A Gα subunit gene is essential for conidiation and potassium efflux but dispensable for pathogenicity of Alternaria alternata on citrus

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A Gα subunit gene is essential for conidiation and potassium efflux but dispensable for pathogenicity of Alternaria alternata on citrus

Content Provider	Springer Nature Link
Author	Wang, Nan Yi Lin, Ching Hsuan Chung, Kuang Ren
Copyright Year	2009
Abstract	Heterotrimeric G proteins play a profound role in the recognition and transduction of extracellular signals in eukaryotic cells. We characterized the AaGα1 gene, encoding a fungal Class I Gα subunit of the GTP-binding protein, in Alternaria alternata of citrus. Interruption of AaGα1 with a marker gene resulted in fungal transformants producing fewer conidia, becoming hypersensitive to KCl, and displaying elevated lipolytic and pectolytic activities. Expression of a functional copy of AaGα1 in a null mutant restored all altered phenotypes to the wild type. The AaGα1 mutants, whose conidia germinate normally, caused necrotic lesions on citrus indistinguishable from wild type. Application of cAMP, its inhibitors (atropine and theophylline), or 3-isobutyl-1-methylxanthine (IBMX) decreased conidiation in the fungal strains carrying a functional AaGα1. In contrast, conidial formation in the null mutants was restored by dibutyryl-cAMP or by a low concentration of cAMP or theophylline (1 mM). Unlike the oxidative stress-responsive AaAP1 transcription activator or the AaFUS3 mitogen-activated protein kinase (MAPK), AaGα1 is not required for cellular resistance to oxidative, osmotic, or chemical stress. AaFUS3 has also been demonstrated to be essential for conidial formation, suggesting a possible interaction between AaGα1 and AaFUS3 during conidiation even though expression of AaGα1 was not affected by AaFUS3 and vice versa. Inactivation of AaGα1 suppressed accumulation of transcripts of the AaAP1 gene and the AaHSK1 gene encoding a histidine kinase. These are novel features that have not been previously characterized to be associated with the GTP-binding protein.
Starting Page	43
Ending Page	51
Page Count	9
File Format	PDF
ISSN	01728083
Journal	Current Genetics
Volume Number	56
Issue Number	1
e-ISSN	14320983
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-12-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cAMP G protein Ion channel MAP kinase Necrotroph Signaling Proteomics Plant Sciences Cell Biology Biochemistry Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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A Gα subunit gene is essential for conidiation and potassium efflux but dispensable for pathogenicity of Alternaria alternata on citrus