NDLI: The constitutive AHSB4 promoter—a novel component of the Arxula adeninivorans-based expression platform

Content Provider	Springer Nature Link
Author	Wartmann, T. Bellebna, C. Böer, E. Bartelsen, O. Gellissen, G. Kunze, G.
Copyright Year	2003
Abstract	An Arxula adeninivorans-AHSB4 gene, encoding histone H4, was isolated and characterized. The gene includes a coding sequence of 363 bp disrupted by a 51-bp intron, similar to the situation in other fungal H4 genes. The identity of the gene was confirmed by the high degree of homology of the derived amino acid sequence with that of other H4 histones. The gene is strongly and constitutively expressed, maintaining this expression profile under salt-stress conditions. The AHSB4 promoter was tested for suitability in heterologous gene expression using genes encoding the intracellular green fluorescent protein and the secreted human serum albumin (HSA) for assessment. Plasmids incorporating respective expression cassettes were used to transform the host strain A. adeninivorans LS3, which forms budding cells at 30 °C, and strain 135, which forms mycelia under these conditions. Transformants of both types were found to harbor a single copy of the heterologous DNA. Strong constitutive expression was observed during culture in salt-containing and salt-free media, as expected from the expression profile of AHSB4. In 200-ml shake-flask cultures, maximal HSA levels of 20 mg l−1 culture medium were achieved. This productivity could be increased to 50 mg l−1 in strains harboring two copies of the expression cassette. The AHSB4 promoter thus provides an attractive component for constitutive heterologous gene expression under salt-free and salt-stress conditions.
Starting Page	528
Ending Page	535
Page Count	8
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	62
Issue Number	5-6
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-05-06
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Large-scale production of tannase using the yeast Arxula adeninivorans

The Arxula adeninivorans ATAL gene encoding transaldolase-gene characterization and biotechnological exploitation

Acid phosphatase production by recombinant Arxula adeninivorans

Identification of a Group‐I Intron Within the 25S rDNA from the Yeast Arxula adeninivorans

Xplor® 2—an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans

AILV1 gene from the yeast Arxula adeninivorans LS3 a new selective transformation marker

Definition of culture conditions for Arxula adeninivorans, a rational basis for studying heterologous gene expression in this dimorphic yeast

Genetic transformation and biotechnological application of the yeast Arxula adeninivorans

Environmental and metabolic parameters affecting the uric acid production of Arxula adeninivorans

The constitutive AHSB4 promoter—a novel component of the Arxula adeninivorans-based expression platform

Similar Documents

Large-scale production of tannase using the yeast Arxula adeninivorans

The Arxula adeninivorans ATAL gene encoding transaldolase-gene characterization and biotechnological exploitation

Acid phosphatase production by recombinant Arxula adeninivorans

Identification of a Group‐I Intron Within the 25S rDNA from the Yeast Arxula adeninivorans

Xplor® 2—an optimized transformation/expression system for recombinant protein production in the yeast Arxula adeninivorans

AILV1 gene from the yeast Arxula adeninivorans LS3 ­ a new selective transformation marker

Definition of culture conditions for Arxula adeninivorans, a rational basis for studying heterologous gene expression in this dimorphic yeast

Genetic transformation and biotechnological application of the yeast Arxula adeninivorans

Environmental and metabolic parameters affecting the uric acid production of Arxula adeninivorans

The constitutive AHSB4 promoter—a novel component of the Arxula adeninivorans-based expression platform

AILV1 gene from the yeast Arxula adeninivorans LS3 a new selective transformation marker