NDLI: Extensive hydrolysis of phosphonates as unexpected behaviour of the known His6-organophosphorus hydrolase

Content Provider	Springer Nature Link
Author	Lyagin, Ilya V. Andriava, Mariia S. Efremenko, Elena N.
Copyright Year	2016
Abstract	The catalytic activity of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) in hydrolytic reactions of methylphosphonic acid (MPA) and its monoesters and diesters being decomposition products of R-VX was demonstrated for the first time. The catalytic constants of enzyme in such reactions were determined. The mechanism of C–P bond cleavage in the MPA by His6-OPH was proposed. Such reaction was estimated to be carried out with the soluble and nanocapsulated forms of His6-OPH. His6-OPH was demonstrated to be capable of degrading the key organophosphorus components of reaction masses (RMs) that are produced by the chemical detoxification of R-VX and RMs are multi-substrate mixtures for this enzyme. The kinetic model describing the behaviour of His6-OPH in RMs was proposed and was shown to adequately fit experimental points during degradation of the real samples of RMs.
Starting Page	5829
Ending Page	5838
Page Count	10
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	100
Issue Number	13
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-03-02
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Organophosphorus compound Phosphonate Mechanism Enzymatic degradation Hexahistidine-tagged organophosphorus hydrolase Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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