NDLI: Characterization of EST3: a metagenome-derived esterase with suitable properties for biotechnological applications

Content Provider	Springer Nature Link
Author	Maester, Thaís Carvalho Pereira, Mariana Rangel Machado Sierra, E. G. Balan, Andrea Lemos, Eliana Gertrudes
Copyright Year	2016
Abstract	Metagenomic libraries from diverse environments have been extensive sources of many lipases and esterases; nevertheless, most of these enzymes remain biochemically uncharacterized. We previously built a metagenomic fosmid library from a microbial consortium specialized for diesel oil degradation and tested it for lipolytic activity. In the present study, we identified the PL14.H10 clone that was subcloned and sequenced, which enabled the identification of the EST3 protein. This enzyme exhibited 74 % amino acid identity with the uncharacterized alpha/beta hydrolase from Parvibaculum lavamentivorans [GenBank: WP012110575.1] and was classified into lipolytic enzyme family IV. Biochemical characterization revealed that EST3 presents high activity in a wide range of temperature with highest activity from 41 to 45 °C. Also, this thermostable esterase acts from mild acidic to alkaline conditions with an optimum pH of 6.0. The enzyme exhibited activity against p-nitrophenyl esters of different chain lengths and highest catalytic efficiency against p-nitrophenyl caprylate. The activity of the protein was increased in the presence of 0.5 mM of Mn+2, Li+, EDTA, and 1 % of CTAB and exhibited half of the activity in the presence of 10 % methanol and ethanol. Moreover, the homology model of EST3 was built and compared to other esterases, revealing a substrate channel that should fit a wide range of substrates. Taken together, the data presented in this work reveal the unique and interesting characteristics of EST3 that might be explored for further use in biotechnological applications.
Starting Page	5815
Ending Page	5827
Page Count	13
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	100
Issue Number	13
e-ISSN	14320614
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-02-26
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Lipase Esterase Family IV Microbial consortium pNP esters Microbiology Microbial Genetics and Genomics Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Identification and characterization of novel poly(dl-lactic acid) depolymerases from metagenome

Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome

Biocatalytic synthesis of ascorbyl esters and their biotechnological applications

Isolation and characterization of novel lipases/esterases from a bovine rumen metagenome

A new esterase EstD2 isolated from plant rhizosphere soil metagenome

Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome

Mannanases: microbial sources, production, properties and potential biotechnological applications

Characterization of Novel Family IV Esterase and Family I.3 Lipase from an Oil-Polluted Mud Flat Metagenome

Isolation and characterization of a family VII esterase derived from alluvial soil metagenomic library

Characterization of EST3: a metagenome-derived esterase with suitable properties for biotechnological applications

Similar Documents

Identification and characterization of novel poly(dl-lactic acid) depolymerases from metagenome

Isolation and characterization of cold-active family VIII esterases from an arctic soil metagenome

Biocatalytic synthesis of ascorbyl esters and their biotechnological applications

Isolation and characterization of novel lipases/esterases from a bovine rumen metagenome

A new esterase EstD2 isolated from plant rhizosphere soil metagenome

Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome

Mannanases: microbial sources, production, properties and potential biotechnological applications

Characterization of Novel Family IV Esterase and Family I.3 Lipase from an Oil-Polluted Mud Flat Metagenome

Isolation and characterization of a family VII esterase derived from alluvial soil metagenomic library

Characterization of EST3: a metagenome-derived esterase with suitable properties for biotechnological applications