NDLI: Metabolic changes underlying the higher accumulation of glutathione in Saccharomyces cerevisiae mutants

Content Provider	Springer Nature Link
Author	Nisamedtiv, Ildar Kevvai, Kaspar Orumets, Kerti Arike, Liisa Sarand, Inga Korhola, Matti Paalme, Toomas
Copyright Year	2010
Abstract	Molecular mechanisms leading to glutathione (GSH) over-accumulation in a Saccharomyces cerevisiae strain produced by UV irradiation-induced random mutagenesis were studied. The mutant accumulated GSH but also cysteine and γ-glutamylcysteine in concentrations that were several fold higher than in its wild-type parent strain under all studied cultivation conditions (chemostat, fed-batch, and turbidostat). Transcript analyses along with shotgun proteome quantification indicated a difference in the expression of a number of genes and proteins, the most pronounced of which were several fold higher expression of CYS3, but also that of GSH1 and its transcriptional activator YAP1. This together with the higher intracellular cysteine concentration is most likely the primary factor underlying GSH over-accumulation in the mutant. Comparative sequencing of GSH1 and the fed-batch experiments with continuous cysteine addition demonstrated that the feedback inhibition of Gsh1p by GSH was still operational in the mutant.
Starting Page	1029
Ending Page	1037
Page Count	9
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	89
Issue Number	4
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-10-30
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Saccharomyces cerevisiae Glutathione Cysteine GSH1 CYS3 YAP1 Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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