NDLI: Specific adsorption of tungstate by cell surface display of the newly designed ModE mutant

Content Provider	Springer Nature Link
Author	Kuroda, Kouichi Nishitani, Takashi Ueda, Mitsuyoshi
Copyright Year	2012
Abstract	By cell surface display of ModE protein that is a transcriptional regulator of operons involved in the molybdenum metabolism in Escherichia coli, we have constructed a molybdate-binding yeast (Nishitani et al., Appl Microbiol Biotechnol 86:641–648, 2010). In this study, the binding specificity of the molybdate-binding domain of the ModE protein displayed on yeast cell surface was improved by substituting the amino acids involved in oxyanion binding with other amino acids. Although the displayed S126T, R128E, and T163S mutant proteins adsorbed neither molybdate nor tungstate, the displayed ModE mutant protein (T163Y) abolished only molybdate adsorption, exhibiting the specific adsorption of tungstate. The specificity of the displayed ModE mutant protein (T163Y) for tungstate was increased by approximately 9.31-fold compared to the displayed wild-type ModE protein at pH 5.4. Therefore, the strategy of protein design and its cell surface display is effective for the molecular breeding of bioadsorbents with metal-specific adsorption ability based on a single species of microorganism without isolation from nature.
Starting Page	153
Ending Page	159
Page Count	7
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	96
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-05-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Binding selectivity Molybdate Tungstate ModE Rare metals Yeast cell surface engineering Microbiology Biotechnology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Molecular design of yeast cell surface for adsorption and recovery of molybdenum, one of rare metals

Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering

Recent developments in yeast cell surface display toward extended applications in biotechnology

Engineering of microorganisms towards recovery of rare metal ions

The contribution of Pir protein family to yeast cell surface display

Enhancement of display efficiency in yeast display system by vector engineering and gene disruption

Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain

Development of new tolerant strains to hydrophilic and hydrophobic organic solvents by the yeast surface display methodology

Specific adsorption of tungstate by cell surface display of the newly designed ModE mutant

Similar Documents

Molecular design of yeast cell surface for adsorption and recovery of molybdenum, one of rare metals

Mutant firefly luciferases with improved specific activity and dATP discrimination constructed by yeast cell surface engineering

Recent developments in yeast cell surface display toward extended applications in biotechnology

Engineering of microorganisms towards recovery of rare metal ions

The contribution of Pir protein family to yeast cell surface display

Enhancement of display efficiency in yeast display system by vector engineering and gene disruption

Beyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display

Simultaneous improvement of saccharification and ethanol production from crystalline cellulose by alleviation of irreversible adsorption of cellulase with a cell surface-engineered yeast strain

Development of new tolerant strains to hydrophilic and hydrophobic organic solvents by the yeast surface display methodology

Specific adsorption of tungstate by cell surface display of the newly designed ModE mutant