NDLI: Lead toxicity in Saccharomyces cerevisiae

Content Provider	Springer Nature Link
Author	Heggen, Maarten Martins, Sara Flores, Gisela Soares, Eduardo V.
Copyright Year	2010
Abstract	The effect of Pb on Saccharomyces cerevisiae cell structure and function was examined. Membrane integrity was assessed by the release of UV-absorbing compounds and by the intracellular K+ efflux. No leakage of UV260-absorbing compounds or loss of K+ were observed in Pb (until 1,000 μmol/l) treated cells up to 30 min; these results suggest that plasma membrane seems not to be the immediate and primary target of Pb toxicity. The effect of Pb on yeast metabolism was examined using the fluorescent probe FUN-1 and compared with the ability to reproduce, evaluated by colony-forming units counting. The exposition of yeast cells, during 60 min to 1,000 μmol/l Pb, induces a decrease in the ability to process FUN-1 although the cells retain its proliferation capacity. A more prolonged contact time (120 min) of yeast cells with Pb induces a marked (> 50%) loss of yeast cells metabolic activity and replication competence through a mechanism which most likely requires protein synthesis.
Starting Page	1355
Ending Page	1361
Page Count	7
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	88
Issue Number	6
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-09-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cell membrane FUN-1 Lead Metal toxicity Yeast viability Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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