NDLI: Ergosterol production from molasses by genetically modified Saccharomyces cerevisiae

Content Provider	Springer Nature Link
Author	He, Xiuping Guo, Xuena Liu, Nan Zhang, Borun
Copyright Year	2007
Abstract	Ergosterol is an economically important metabolite produced by fungi. Recombinant Saccharomyces cerevisiae YEH56(pHXA42) with increased capacity of ergosterol formation was constructed by combined overexpression of sterol C-24(28) reductase and sterol acyltransferase in the yeast strain YEH56. The production of ergosterol by this recombinant strain using cane molasses (CM) as an inexpensive carbon source was investigated. An ergosterol content of 52.6 mg/g was obtained with 6.1 g/l of biomass from CM medium containing 60 g/l of total sugar in 30 h in shake flask. The ergosterol yield was enhanced through the increasing cell biomass by supplementation of urea to a concentration of 6 g/l in molasses medium. Fermentation was performed in 5-l bioreactor using the optimized molasses medium. In batch fermentation, the effect of agitation velocity on ergosterol production was examined. The highest ergosterol yield was obtained at 400 rpm that increased 60.4 mg/l in comparison with the shake flask culture. In fed-batch fermentation, yeast cells were cultivated, firstly, in the starting medium containing molasses with 20 g/l of total sugar, 1.68 g/l of phosphate acid, and 6 g/l of urea (pH 5.4) for 5 h, then molasses containing 350 g/l of total sugar was fed exponentially into the bioreactor to keep the ethanol level in the broth below 0.5%. After 40 h of cultivation, the ergosterol yield reached 1,707 mg/l, which was 3.1-fold of that in the batch fermentation.
Starting Page	55
Ending Page	60
Page Count	6
File Format	PDF
ISSN	01757598
Journal	Applied Microbiology and Biotechnology
Volume Number	75
Issue Number	1
e-ISSN	14320614
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-01-16
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Ergosterol Combined overexpression Sterol C-24(28) reductase Sterol acyltransferase Molasses Fed-batch fermentation Microbial Genetics and Genomics Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Overexpression of a sterol C-24(28) reductase increases ergosterol production in Saccharomyces cerevisiae

Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation

Ethanol production from sweet sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae

Expression of a mutated SPT15 gene in Saccharomyces cerevisiae enhances both cell growth and ethanol production in microaerobic batch, fed-batch, and simultaneous saccharification and fermentations

Genetically modified Saccharomyces cerevisiae for one-step fermentation of bioalcohol using corncob as sole carbon source

Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae

1,3-Propanediol production in a two-step process fermentation from renewable feedstock

Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast

Batch ethanol production from cassava (Manihot esculenta Crantz.) flour using Saccharomyces cerevisiae cells immobilized in calcium alginate

Ergosterol production from molasses by genetically modified Saccharomyces cerevisiae

Similar Documents

Overexpression of a sterol C-24(28) reductase increases ergosterol production in Saccharomyces cerevisiae

Comparative proteome analysis of robust Saccharomyces cerevisiae insights into industrial continuous and batch fermentation

Ethanol production from sweet sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae

Expression of a mutated SPT15 gene in Saccharomyces cerevisiae enhances both cell growth and ethanol production in microaerobic batch, fed-batch, and simultaneous saccharification and fermentations

Genetically modified Saccharomyces cerevisiae for one-step fermentation of bioalcohol using corncob as sole carbon source

Repeated batch ethanolic fermentation of very high gravity medium by immobilized Saccharomyces cerevisiae

1,3-Propanediol production in a two-step process fermentation from renewable feedstock

Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast

Batch ethanol production from cassava (Manihot esculenta Crantz.) flour using Saccharomyces cerevisiae cells immobilized in calcium alginate

Ergosterol production from molasses by genetically modified Saccharomyces cerevisiae