NDLI: Genetically modified Saccharomyces cerevisiae for one-step fermentation of bioalcohol using corncob as sole carbon source

Content Provider	Springer Nature Link
Author	Song, Huiting Liu, Jiashu Liu, Yang Leng, Huan Li, Xu Zhao, Yong Yang, Xiaosong Jiang, Zhengbing
Copyright Year	2013
Abstract	The corncob is an important biomass for bioalcohol production. However, there is a minor but complicated pretreatment process before it is used for bioalcohol fermentation. In this study, three genetically modified Saccharomyces cerevisiae Y33 strains containing endoglucanase (EG), cellobiohydrolase (CBH), and β-glucosidase (BG) genes were constructed. A one-step fermentation process was carried out with the recombinants using corncob as the sole carbon source. In a 3-L fermentation system, the concentration of alcohol reached 2.02 g/L and the concentration of glycerine reached 0.85 g/L after 96 h. The results prove that corncob powder can be utilized effectively by genetically modified Saccharomyces cerevisiae without any chemical pretreatment. The mixed recombinant Saccharomyces cerevisiae cells show effective synergism in the one-step fermentation system. It is feasible that corncob can be used as the sole carbon source in bioalcohol production with a one-step fermentation process.
Starting Page	781
Ending Page	785
Page Count	5
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	64
Issue Number	2
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-08-18
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cellulase Genetically modified yeast Corncob cellulose One-step fermentation Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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