NDLI: Amino acid 95 causes strong alteration of peptide position PΩ in HLA-B*41 variants

Content Provider	Springer Nature Link
Author	Bade Doeding, Christina DeLuca, David S. Seltsam, Axel Blasczyk, Rainer Eiz Vesper, Britta
Copyright Year	2007
Abstract	There have been several attempts over the years to identify positions in the peptide-binding region (PBR) of human leukocyte antigens (HLA) that influence the specificity of bound amino acids (AAs) at each position in the peptide. Originally, six pockets (A–F) were defined by calculating the surface area of the PBR on the crystal structure of HLA-A2 molecules. More recent crystallographic analyses of a variety of HLA alleles have led to broader pocket definitions. In this study, we examined the peptide-binding specificity of HLA-B41 alleles and compared our results with the available pocket definitions. By generating recombinant HLA-B molecules and studying the eluted peptides by mass spectrometry and pool sequencing, we detected two different PΩ peptide motifs within the B41 group: Leu vs Val/Pro. Specificity was dependent on the presence of Leu (B4102, B4103, and B4104) vs Trp (B4101, B4105, and B4106) at AA position 95 in the HLA molecule, whose impact on PΩ has been a subject of controversy in current pocket definitions. In contrast, the Arg97Ser mutation did not affect pocket F binding specificity in B41 subtypes although residue 97 was previously identified as a modulator of peptide binding for several HLA class I alleles. According to most pocket definitions, this study shows that the Asn80Lys substitution in B4105 impels the peptide’s PΩ anchor toward more promiscuity. Our sequencing results of peptides eluted from HLA-B*41 variants demonstrate the limitations of current pocket definitions and underline the need for an extended peptide motif database for improved understanding of peptide–major histocompatibility complex interactions.
Starting Page	253
Ending Page	259
Page Count	7
File Format	PDF
ISSN	00937711
Journal	Immunogenetics
Volume Number	59
Issue Number	4
e-ISSN	14321211
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-02-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Peptide-binding motif PΩ position Pocket definition Cell Biology Allergology Immunology
Content Type	Text
Resource Type	Article
Subject	Genetics Immunology

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