NDLI: Genetic Evidence for Reproductive Isolation Among Sympatric Epichloë Endophytes as Inferred from Newly Developed Microsatellite Markers

Content Provider	Springer Nature Link
Author	Schirrmann, Melanie K. Zoller, Stefan Fior, Simone Leuchtmann, Adrian
Copyright Year	2014
Abstract	Reproductive isolation is central to the maintenance of species, and especially in sympatry, effective barriers to prevent interspecific crosses are expected. Host specificity is thought to constitute an effective mechanism for the formation of barriers in different genera of Fungi, but evidence for endophytes is so far lacking. Sexual Epichloë species (Ascomycota, Clavicipitaceae) represent an ideal study system to investigate the mechanisms underlying speciation as mediated by host specificity because they include species complexes with several host-specific taxa. Here, we studied genetic differentiation of three host-specific Epichloë species using microsatellite markers that were newly in silico identified on the genome of Epichloë poae. Among these, 15 were experimentally tested and applied to study an extensive sampling of isolates representing Epichloë typhina infecting Dactylis glomerata and Epichloë clarkii infecting Holcus lanatus from a site with sympatric populations in Switzerland, as well as a reduced sampling of E. poae infecting Poa nemoralis to create a three-taxon dataset. Both principal coordinate analysis and Bayesian clustering algorithm showed three genetically distinct groups representing the three host-specific species. High pairwise F ST values among the three species, as well as sequencing data of the tefA gene revealing diagnostic single nucleotide polymorphisms (SNPs), further support the hypothesis of genetic discontinuities among the taxa. These results provide genotypic evidence of the maintenance of reproductive isolation of the species in a context of sympatry. In silico testing of 885 discovered microsatellites on the genome of Epichloë festucae extend their applicability to a wider taxonomic range of Epichloë.
Starting Page	51
Ending Page	60
Page Count	10
File Format	PDF
ISSN	00953628
Journal	Microbial Ecology
Volume Number	70
Issue Number	1
e-ISSN	1432184X
Language	English
Publisher	Springer US
Publisher Date	2014-12-28
Publisher Place	New York
Access Restriction	Subscribed
Subject Keyword	Endophytes Microsatellites Population structure Reproductive isolation Sympatry Microbiology Ecology Microbial Ecology Geoecology/Natural Processes Nature Conservation Water Quality/Water Pollution
Content Type	Text
Resource Type	Article
Subject	Soil Science Ecology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Infection with a Shoot-Specific Fungal Endophyte (Epichloë) Alters Tall Fescue Soil Microbial Communities

Aboveground Epichloë coenophiala–Grass Associations Do Not Affect Belowground Fungal Symbionts or Associated Plant, Soil Parameters

Microbial Ecology Update

Evidence of Novel Phylogenetic Lineages of Methanogenic Archaea from Hypersaline Microbial Mats

Geographical and Temporal Changes of Foliar Fungal Endophytes Associated with the Invasive Plant Ageratina adenophora

Fungal Endophytes of Aquatic Macrophytes: Diverse Host-Generalists Characterized by Tissue Preferences and Geographic Structure

Microbial Competition in Reactors with Wall Attachment

Shedding Light on Microbial Predator–Prey Population Dynamics Using a Quantitative Bioluminescence Assay

Land Use Type Significantly Affects Microbial Gene Transcription in Soil

Genetic Evidence for Reproductive Isolation Among Sympatric Epichloë Endophytes as Inferred from Newly Developed Microsatellite Markers

Similar Documents

Infection with a Shoot-Specific Fungal Endophyte (Epichloë) Alters Tall Fescue Soil Microbial Communities

Aboveground Epichloë coenophiala–Grass Associations Do Not Affect Belowground Fungal Symbionts or Associated Plant, Soil Parameters

Microbial Ecology Update

Evidence of Novel Phylogenetic Lineages of Methanogenic Archaea from Hypersaline Microbial Mats

Geographical and Temporal Changes of Foliar Fungal Endophytes Associated with the Invasive Plant Ageratina adenophora

Fungal Endophytes of Aquatic Macrophytes: Diverse Host-Generalists Characterized by Tissue Preferences and Geographic Structure

Microbial Competition in Reactors with Wall Attachment

Shedding Light on Microbial Predator–Prey Population Dynamics Using a Quantitative Bioluminescence Assay

Land Use Type Significantly Affects Microbial Gene Transcription in Soil

Genetic Evidence for Reproductive Isolation Among Sympatric Epichloë Endophytes as Inferred from Newly Developed Microsatellite Markers