NDLI: Organochlorine Contaminants in Loggerhead Sea Turtle Blood: Extraction Techniques and Distribution Among Plasma and Red Blood Cells

Content Provider	Springer Nature Link
Author	Keller, J. M. Kucklick, J. R. McClellan Green, P. D.
Copyright Year	2004
Abstract	Few studies have described the organochlorine (OC) contaminant concentrations found in sea turtle tissues. These studies have relied on the opportunistic sampling of either eggs or tissues from stranded carcasses. In this study, the use of whole blood samples as well as both blood components (plasma and red blood cells) were examined as a non-destructive alternative for monitoring OCs in free-ranging loggerhead sea turtles (Caretta caretta). Blood samples were collected from juvenile loggerhead sea turtles (n = 12) captured in Core Sound, North Carolina, USA and analyzed for 55 polychlorinated biphenyl (PCB) congeners and 24 OC pesticides by gas chromatography with electron capture detection and mass spectrometry. Using pooled loggerhead sea turtle whole blood, three different liquid:liquid extraction techniques were compared. Results were similar in terms of recovery of internal standards, lipids, and OC concentrations. An extraction technique, employing formic acid and 1:1 methyl-tert-butyl-ether:hexane, was found to be satisfactory. This method was applied to the extraction of OCs from whole blood, plasma, and red blood cell (RBC) samples from five loggerhead sea turtles. Plasma contained the highest OC concentrations on a wet mass basis, followed by whole blood and RBCs. The majority of each OC compound was found in the plasma rather than the RBCs, suggesting that OC compounds preferentially partition into the plasma. On average (SD), 89.4% (3.1%) of total PCBs, 83.4% (11.9%) of total chlordanes, 74.3% (15.1%) of mirex, 72.6% (4.8%) of total DDTs, and 80.1% (16.6%) of dieldrin were found in the plasma. The concentrations of total PCBs, mirex, total chlordanes, and total DDTs measured in both components of the blood significantly correlated to those in whole blood. These are the first reported OC concentrations in sea turtle blood. They were found to be similar to previously reported levels in blood components of humans and of reptiles from relatively clean sites, but lower than those measured in blood of fish-eating birds and marine mammals. The results indicate that blood, preferably plasma, can be used to detect and monitor OC contaminants in loggerhead sea turtles.
Starting Page	254
Ending Page	264
Page Count	11
File Format	PDF
ISSN	00904341
Journal	Archives of Environmental Contamination and Toxicology
Volume Number	46
Issue Number	2
e-ISSN	14320703
Language	English
Publisher	Springer-Verlag
Publisher Date	2004-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Pollution Health, Toxicology and Mutagenesis Medicine Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Organochlorine Contaminants in Loggerhead Sea Turtle Blood: Extraction Techniques and Distribution Among Plasma and Red Blood Cells

Comparative Study of Organohalogen Contamination Between Two Populations of Eastern Atlantic Loggerhead Sea Turtles (Caretta caretta)

Effects of organochlorine contaminants on loggerhead sea turtle immunity: comparison of a correlative field study and in vitro exposure experiments. Environ. Health perspect (2006).

Effects of organochlorine contaminants on loggerhead sea turtle immunity: comparison of a correlative field study and in vitro exposure experiments.

Organochlorine Pesticide Contaminants in Human Milk Samples Collected in Tebriz (Iran)

Polychlorinated Naphthalenes and Other Organochlorine Contaminants in Swedish Human Milk, 1972–1992

Bioaccumulation of Organochlorine Contaminants in Bowhead Whales (Balaena mysticetus) from Barrow, Alaska

Organochlorine and Heavy Metal Contaminants in Wild Mink in Western Northwest Territories, Canada

Distribution and enantiomeric profiles of organochlorine pesticides in surface sediments from the Bering Sea, Chukchi Sea and adjacent Arctic areas.

Organochlorine Contaminants in Loggerhead Sea Turtle Blood: Extraction Techniques and Distribution Among Plasma and Red Blood Cells

Similar Documents

Organochlorine Contaminants in Loggerhead Sea Turtle Blood: Extraction Techniques and Distribution Among Plasma and Red Blood Cells

Comparative Study of Organohalogen Contamination Between Two Populations of Eastern Atlantic Loggerhead Sea Turtles (Caretta caretta)

Effects of organochlorine contaminants on loggerhead sea turtle immunity: comparison of a correlative field study and in vitro exposure experiments. Environ. Health perspect (2006).

Effects of organochlorine contaminants on loggerhead sea turtle immunity: comparison of a correlative field study and in vitro exposure experiments.

Organochlorine Pesticide Contaminants in Human Milk Samples Collected in Tebriz (Iran)

Polychlorinated Naphthalenes and Other Organochlorine Contaminants in Swedish Human Milk, 1972–1992

Bioaccumulation of Organochlorine Contaminants in Bowhead Whales (Balaena mysticetus) from Barrow, Alaska

Organochlorine and Heavy Metal Contaminants in Wild Mink in Western Northwest Territories, Canada

Distribution and enantiomeric profiles of organochlorine pesticides in surface sediments from the Bering Sea, Chukchi Sea and adjacent Arctic areas.

Organochlorine Contaminants in Loggerhead Sea Turtle Blood: Extraction Techniques and Distribution Among Plasma and Red Blood Cells