NDLI: High-throughput β-glucan analyses and their relationship with beer filterability

Content Provider	Springer Nature Link
Author	Kupetz, M. Aumer, J. Harms, D. Zarnkow, M. Sacher, B. Becker, T.
Copyright Year	2016
Abstract	Beer filterability is strongly related to the amount and molar mass distribution of β-glucan. Different commercial quantification methods yield different β-glucan concentrations and beer filterability. In order to acquire detailed information on the measureable molar mass and its impact on beer filtration, six high-throughput methods were developed and analysed. These methods involve three calibration standards (Skandinavisk Bryggeri Laboratorium standard, SBL; Megazyme standard for continuous flow analyser, CFA; and 650 kDa standard) and two dyes (Calcofluor and Congo red). Significant correlation was observed between cellulose nitrate membrane filtration and fluorometrically determined β-glucan content (SBL calibration: r = 0.604, P < 0.001; CFA calibration: r = 0.687, P < 0.001). However, no correlation was observed with the colorimetric methods. Polyethersulphone membrane filtration correlated significantly with specific viscosity (r = 0.710, P < 0.001) and fluorometrically determined β-glucan content (CFA calibration: r = 0.586, P < 0.001). However, there was no correlation between the filtration performance of diatomaceous earth and the established beer properties. No correlation between the developed colorimetric and fluorometric methods was found using the SBL and CFA standards. However, a significant correlation was observed with the 650 kDa standard (r = 0.836, P < 0.001). A wide variation, especially between the dyes, was observed in the measurable β-glucan molar mass distribution determined using field-flow fractionation. Our results demonstrate the impact of method parameters on β-glucan concentration. Thus, known β-glucan thresholds regarding filterability must be reconsidered. We conclude that a valid β-glucan quantification method can provide reliable information on beer filterability.
Starting Page	341
Ending Page	351
Page Count	11
File Format	PDF
ISSN	14382377
Journal	European Food Research and Technology
Volume Number	243
Issue Number	2
e-ISSN	14382385
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2016-07-21
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Filterability Carbohydrates Barley Photometry Food Science Analytical Chemistry Biotechnology Agriculture Forestry
Content Type	Text
Resource Type	Article
Subject	Industrial and Manufacturing Engineering Chemistry Biochemistry Food Science Biotechnology

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