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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kim, Heon Woong Ko, Ho Cheol Baek, Hyung Jin Cho, Soo Muk Jang, Hwan Hee Lee, Young Min Kim, Jung Bong |
| Copyright Year | 2016 |
| Abstract | In the present investigation, glucosinolate content was identified and quantified in 210 accessions of Korean leaf mustard (Brassica juncea var. integrifolia) by a liquid chromatography (LC) with electrospray ionization (ESI) coupled with a positive-mode ion trap tandem mass spectrometry (LC–ESI–MS/MS). Eleven individual GSLs including two new compounds were identified in the accessions using desulfo-glucosinolates (DS-GSLs) LC–MS library, and they were elucidated by interpreting the fragmentation data ([M + H-glucose]+) obtained. The newly identified GSLs contained aliphatic and hydroxyl functional group in their back bone were named as 1-ethyl-2-hydroxyethyl DS-GSLs (glucosisautricin) or 2-hydroxy-2-methylpropyl DS-GSLs (glucoconringiin) and 3,4,5-trimethoxybenzyl DS-GSLs, respectively. Among all tested accessions, the total DS-GSLs content was found to be varied between 5.3 and 23.2 mg/g dry weight (DW) with an average of 13.0 mg/g DW in the germplasm. Each individual DS-GSLs component was found in decreasing order of sinigrin (41.7 %) >glucoiberverin (21.7 %) >gluconasturtiin (12.6 %) >glucobarbarin (10.0 %) >glucoiberin (5.1 %) >glucocheirolin (3.6 %) >glucobrassicanapin (2.6 %) >gluconapin (2.1 %), and >glucobrassicin (0.6 %). Interestingly, sinigrin and gluconasturtiin were present in higher content, and progoitrin was not detected significantly in the germplasm of Korean leaf mustard. In particular, accession K046197 (purple) was found to show highest total DS-GSL content (23.2 mg/g DW). |
| Starting Page | 1479 |
| Ending Page | 1484 |
| Page Count | 6 |
| File Format | |
| ISSN | 14382377 |
| Journal | European Food Research and Technology |
| Volume Number | 242 |
| Issue Number | 9 |
| e-ISSN | 14382385 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-03-01 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Evaluation Glucosinolates Korean leaf mustard germplasm Brassica juncea var. integrifolia LC–ESI–MS/MS Food Science Analytical Chemistry Biotechnology Agriculture Forestry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Industrial and Manufacturing Engineering Chemistry Biochemistry Food Science Biotechnology |
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