NDLI: Inheritance of resistance to covered smut in barley and development of a tightly linked SCAR marker

Content Provider	Springer Nature Link
Author	Ardiel, G. S. Grewal, T. S. Deberdt, P. Rossnagel, B. G. Scoles, G. J.
Copyright Year	2002
Abstract	Inheritance of resistance to covered smut in the barley line Q21861 was studied using a doubled-haploid population produced by crossing Q21861 with the line SM89010. Based on 3 years of screening in the field and two seasons in the greenhouse, segregation for resistance/susceptibility fits a one-gene ratio, indicating a single major gene for resistance in Q21861. Of 440 random 10-mer primers tested using bulked segregant analysis, one primer (OPJ10) resulted in a reproducible polymorphic band. RAPD marker OPJ10450 co-segregated in repulsion with the covered smut resistance. This marker was converted to a sequence-characterized amplified region (SCAR) marker linked in coupling (5.5 cM) with the covered smut resistant gene in Q21861. The SCAR marker was amplified in the line TR640 which is also resistant to covered smut, but not in the other resistant lines. The SCAR marker will be useful for marker-assisted selection for covered smut in barley breeding programs.
Starting Page	457
Ending Page	464
Page Count	8
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	104
Issue Number	2
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-02-01
Publisher Place	Berlin Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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