NDLI: Identification of AFLP markers linked to a resistance gene against pine needle gall midge in Japanese black pine

Content Provider	Springer Nature Link
Author	Hayashi, E. Kondo, T. Terada, K. Kuramoto, N. Kawasaki, S.
Copyright Year	2003
Abstract	Bulked segregant and AFLP analyses of two mapping populations (R17 × S6 and R17 × S1) were used to identify markers linked to Rpgm, the only known gene responsible for resistance to pine needle gall midge in Pinus thunbergii Parl. Rpgm was found to be bracketed by ACCC/CCTTT 190 on one side at a distance of 6.6 cM and ACGT/CCCGC 250 at 15.3 cM on the other side. The segregation of these markers was analyzed in two other families in order to determine their phase and transferability. One of the two additional resistant parents carried ACCC/CCTTT 190 in the homozygous state while the marker was in coupling (plus marker allele linked with an R allele) in a resistant parent, R17. The marker ACGT/CCCGC 250 was in a repulsion phase in R17 and was not detected in the other two resistant pine trees. Out of four AFLP markers identified, only ACGT/CCAAT 290 was transferable in all resistant trees tested, although its phase was opposite for different trees. These results indicate that in applying those markers to select resistant trees, the phase state of the markers in each resistant tree with respect to Rpgm needs to be considered.
Starting Page	1177
Ending Page	1181
Page Count	5
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	108
Issue Number	6
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-12-09
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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