NDLI: RAPD markers linked to a gene for resistance to pine needle gall midge in Japanese black pine (Pinus thunbergii)

Content Provider	Springer Nature Link
Author	Kondo, T. Terada, K. Hayashi, E. Kuramoto, N. Okamura, M. Kawasaki, H.
Copyright Year	2000
Abstract	Linkage of RAPD markers to a single dominant gene for resistance to pine needle gall midge was investigated in Japanese black pine (Pinus thunbergii). Three primers that generated linked markers were found after 1160 primers were screened by bulked segregant analysis. The distances between the resistance gene, R, and the marker genes OPC06580, OPD01700, and OPAX192100 were 5.1 cM, 6.7 cM and 13.6 cM, respectively. OPC06580 was in coupling phase to R, whereas OPD01700 and OPAX192100 were in repulsion phase to R. A linkage map for a resistant tree was constructed using 96 macrogametophytes. In linkage analysis, 98 out of 127 polymorphic markers were assigned to 17 linkage groups and six linked pairs. The total length of this map was 1469.8 cM, with an average marker density of 15.6 cM. The genome length was estimated to be 2138.3 cM, and the derived linkage map covered 67.5% of the genome. Although the linked markers OPC06580, OPAX192100, and OPD01700, belonged to the same linkage group, no precise positions were found for OPC06580 or OPD01700.
Starting Page	391
Ending Page	395
Page Count	5
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	100
Issue Number	3
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-02-16
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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