NDLI: Selection and molecular characterization of a lipoxygenase-free soybean mutant line induced by gamma irradiation

Content Provider	Springer Nature Link
Author	Lee, Kyung Jun Hwang, Jung Eun Velusamy, Vijayanand Ha, Bo Keun Kim, Jin Baek Kim, Sang Hoon Ahn, Joon Woo Kang, Si Yong Kim, Dong Sub
Copyright Year	2014
Abstract	A lipoxygenase-free soybean mutant line (H70) induced by gamma ray was selected and its detailed information about the lipoxygenase was analyzed by comparison of DNA sequence. Soybean seeds contain three lipoxygenase enzymes, which induce a beany or grassy flavor. The elimination of lipoxygenases can reduce the poor stability and off-flavors of soybean oil and protein products. In this study, we selected a soybean mutant (H70) in which the three lipoxygenases had been mutated using gamma rays. To obtain detailed information about the lipoxygenase, we investigated the sequences of the Lox1, Lox2 and Lox3 genes in H70 compared to the original cultivar, Hwanggum. Comparisons of the sequences of the Lox1 and Lox2 genes in H70 with those in a line with normal lipoxygenase (HG) showed that the mutations in these genes affected a highly conserved group of six histidine residues necessary for enzymatic activity. Lox1 in H70 contained a 74 bp deletion in exon 8, creating a stop codon that prematurely terminates translation. A single point mutation (T-A) in exon 8 of Lox2 changed histidine (H532, one of the iron-binding ligands essential for Lox2 activity) to glutamine. The mutation in the Lox3 gene in H70 was a single-point mutation in exon 6 (A-G), which changed the amino acid from histidine to arginine. This amino acid alteration in Lox3 was located in the N-terminal barrel, which might play a role in molecular recognition during catalysis and/or proteolysis. These results suggest that gene analysis based on DNA sequencing could be useful for elucidating the lipoxygenase content in soybean mutant lines. Additionally, the soybean mutant line selected in this study could be used to develop soybean cultivars with improved flavor.
Starting Page	2405
Ending Page	2413
Page Count	9
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	127
Issue Number	11
e-ISSN	14322242
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-09-05
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Plant Breeding/Biotechnology Plant Genetics & Genomics Agriculture Plant Biochemistry Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

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