NDLI: Identification of Kunitz trypsin inhibitor mutations using SNAP markers in soybean mutant lines

Content Provider	Springer Nature Link
Author	Kim, D. S. Lee, K. J. Kim, J. B. Kim, S. H. Song, J. Y. Seo, Y. W. Lee, B. M. Kang, S. Y.
Copyright Year	2010
Abstract	The Kunitz trypsin inhibitor (KTi) in soybean has several polymorphic types that are controlled by multiple alleles, which behave in a co-dominant fashion. Of these, Tia and Tib, which differ by nine amino acids, are the predominant types. In order to develop a single nucleotide amplified polymorphism (SNAP) marker for the classification of the predominant KTi types, Tia and Tib, and evaluate KTi activities by differing KTi type total 451 soybean mutant lines (M12–M16 generation) were incorporated in this study. Among 451 soybean mutants, 144 and 13 mutant lines showed decreased and increased trypsin inhibitor activity when compared with the original cultivars, respectively. To identify the KTi type, we designed a SNAP marker. Among 451 mutant lines from 12 soybean cultivars and landraces, 8 mutant lines derived from cvs. Baekwoon, Paldal and Suwon115 showed a change in KTi type when compared with the original cultivars using the SNAP marker. Five mutant lines in Suwon115 changed from Tib to Tia, while two mutant lines derived from cv. Baekwoon and one mutant line derived from cv. Paldal were changed from Tia to Tib. These changes of KTi types were confirmed by sequencing of the KTi genes and non-denaturing polyacrylamide gel electrophoresis of the KTi proteins. To identify the effect of KTi activity based on the change in KTi type, we measured the KTi activity using the three cultivars and eight mutant lines that showed changes in KTi type. Two mutant lines (BW-1 and 7-2) derived from cv. Baekwoon and one mutant line (PD-5-10) from cv. Paldal that changed from Tia to Tib showed lower activity than the original cultivar. In cv. Suwon115, five mutant lines that changed from Tib to Tia showed higher activity than the original cultivar. These results indicate that the designed SNAP marker was capable of identifying the KTi type and that Tia activity was higher than Tib activity in soybean.
Starting Page	751
Ending Page	760
Page Count	10
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	121
Issue Number	4
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-05-06
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Biochemistry Plant Biochemistry Agriculture Plant Genetics & Genomics Plant Breeding/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Allelic differentiation of Kunitz trypsin inhibitor in wild soybean (Glycine soja)

Genome-wide identification of intron fragment insertion mutations and their potential use as SCAR molecular markers in the soybean

Identification of a novel gene, H34, in wheat using recombinant inbred lines and single nucleotide polymorphism markers

High-throughput identification of genetic markers using representational oligonucleotide microarray analysis

Identification of QTL for increased fibrous roots in soybean

Gene expression profiling of resistant and susceptible soybean lines infected with soybean cyst nematode

Identification of a soybean rust resistance gene in PI 567104B

Identification and verification of QTLs for agronomic traits using wild barley introgression lines

Identification and molecular mapping of two soybean aphid resistance genes in soybean PI 587732

Identification of Kunitz trypsin inhibitor mutations using SNAP markers in soybean mutant lines

Similar Documents

Allelic differentiation of Kunitz trypsin inhibitor in wild soybean (Glycine soja)

Genome-wide identification of intron fragment insertion mutations and their potential use as SCAR molecular markers in the soybean

Identification of a novel gene, H34, in wheat using recombinant inbred lines and single nucleotide polymorphism markers

High-throughput identification of genetic markers using representational oligonucleotide microarray analysis

Identification of QTL for increased fibrous roots in soybean

Gene expression profiling of resistant and susceptible soybean lines infected with soybean cyst nematode

Identification of a soybean rust resistance gene in PI 567104B

Identification and verification of QTLs for agronomic traits using wild barley introgression lines

Identification and molecular mapping of two soybean aphid resistance genes in soybean PI 587732

Identification of Kunitz trypsin inhibitor mutations using SNAP markers in soybean mutant lines