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| Content Provider | Springer Nature Link |
|---|---|
| Author | Dudas, Brigitta Jenes, Barnabas Kiss, Gyorgy Botond Maliga, Pal |
| Copyright Year | 2012 |
| Abstract | We report here the isolation of spectinomycin-resistant mutants in cultured cells of Medicago sativa line RegenSY-T2. Spectinomycin induces bleaching of cultured alfalfa cells due to inhibition of protein synthesis on the prokaryotic type 70S plastid ribosomes. Spontaneous mutants resistant to spectinomycin bleaching were identified by their ability to form green shoots on plant regeneration medium containing selective spectinomycin concentrations in the range of 25–50 mg/l. Sequencing of the plastid rrn16 gene revealed that spectinomycin resistance is due to mutations in a conserved stem structure of the 16S rRNA. Resistant plants transferred to the greenhouse developed normally and produced spectinomycin-resistant seed progeny. In light of their absence in soybean, a related leguminous plant, the isolation of spectinomycin-resistant mutants in M. sativa was unexpected. The new mutations are useful for the study of plastid inheritance, as demonstrated by detection of predominantly paternal plastid inheritance in the RegenSY-T2 × Szapko57 cross, and can be used as selective markers in plastid transformation vectors to obtain cisgenic plants. |
| Starting Page | 1517 |
| Ending Page | 1523 |
| Page Count | 7 |
| File Format | |
| ISSN | 00405752 |
| Journal | Theoretical and Applied Genetics |
| Volume Number | 125 |
| Issue Number | 7 |
| e-ISSN | 14322242 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-07-13 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Agriculture Plant Biochemistry Biotechnology Plant Genetics & Genomics Biochemistry Plant Breeding/Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Biotechnology Agronomy and Crop Science |
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