NDLI: Dehydrin variants associated with superior freezing tolerance in alfalfa (Medicago sativa L.)

Content Provider	Springer Nature Link
Author	Rémus Borel, Wilfried Castonguay, Yves Cloutier, Jean Michaud, Réal Bertrand, Annick Desgagnés, Réjean Laberge, Serge
Copyright Year	2009
Abstract	A cDNA (msaCIG) encoding a cold-inducible Y2K4 dehydrin in alfalfa (Medicago sativa spp. sativa) was shown to share extensive homology with sequences from other species and subspecies of Medicago. Differences were mainly the result of the occurrence of large indels, amino acids substitutions/deletions and sequence duplications. Using a combination of a bulk segregant analysis and RFLP hybridization, we uncovered an msaCIG polymorphism that increases in frequency in response to recurrent selection for superior freezing tolerance. Progenies from crosses between genotypes with (D+) or without (D−) the polymorphic dehydrin significantly differed in their tolerance to subfreezing temperatures. Based on the msaCIG sequence, we looked for intragenic variations that could be associated to the polymorphism detected on Southern blots. Amplifications with primers targeting the 3′ half side of msaCIG revealed fragment size variations between pools of genotypes with (+) or without (−) the polymorphism. Three major groups of amplicons of ≈370 nt (G1), 330 nt (G2), and 290 nt (G3) were distinguished. The G2 group was more intensively amplified in pools of genotypes with the polymorphic dehydrin and was associated to a superior freezing tolerance phenotype. Sequences analysis revealed that size variation in the 3′ half was attributable to the variable occurrence of large indels. Single amino acid substitutions and/or deletions caused major differences in the prediction of the secondary structure of the polypeptides. The identification of dehydrin variants associated to superior freezing tolerance paves the way to the development of functional markers and the fixation of favorable alleles in various genetic backgrounds.
Starting Page	1163
Ending Page	1174
Page Count	12
File Format	PDF
ISSN	00405752
Journal	Theoretical and Applied Genetics
Volume Number	120
Issue Number	6
e-ISSN	14322242
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-12-29
Publisher Place	Berlin/Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biotechnology Biochemistry Plant Biochemistry Agriculture Plant Genetics & Genomics Plant Breeding/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biotechnology Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

SRAP polymorphisms associated with superior freezing tolerance in alfalfa (Medicago sativa spp. sativa)

Characterization of two novel cold-inducible K3 dehydrin genes from alfalfa (Medicago sativa spp. sativa L.)

Intron-length polymorphism identifies a Y2K4 dehydrin variant linked to superior freezing tolerance in alfalfa

Erratum to: Intron-length polymorphism identifies a Y2K4 dehydrin variant linked to superior freezing tolerance in alfalfa

Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.)

An expressed sequence tag SSR map of tetraploid alfalfa (Medicago sativa L.)

Genetic diversity among alfalfa (Medicago sativa) cultivars coming from a breeding program, using SSR markers

Inferring population structure and genetic diversity of broad range of wild diploid alfalfa (Medicago sativa L.) accessions using SSR markers

A method to measure genetic distance between allogamous populations of alfalfa (Medicago sativa) using RAPD molecular markers

Dehydrin variants associated with superior freezing tolerance in alfalfa (Medicago sativa L.)

Similar Documents

SRAP polymorphisms associated with superior freezing tolerance in alfalfa (Medicago sativa spp. sativa)

Characterization of two novel cold-inducible K3 dehydrin genes from alfalfa (Medicago sativa spp. sativa L.)

Intron-length polymorphism identifies a Y2K4 dehydrin variant linked to superior freezing tolerance in alfalfa

Erratum to: Intron-length polymorphism identifies a Y2K4 dehydrin variant linked to superior freezing tolerance in alfalfa

Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.)

An expressed sequence tag SSR map of tetraploid alfalfa (Medicago sativa L.)

Genetic diversity among alfalfa (Medicago sativa) cultivars coming from a breeding program, using SSR markers

Inferring population structure and genetic diversity of broad range of wild diploid alfalfa (Medicago sativa L.) accessions using SSR markers

A method to measure genetic distance between allogamous populations of alfalfa (Medicago sativa) using RAPD molecular markers

Dehydrin variants associated with superior freezing tolerance in alfalfa (Medicago sativa L.)