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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kassiri, Zamaneh Hajjar, Roger Backx, Peter H. |
| Copyright Year | 2002 |
| Abstract | We have previously reported that Kv1.4, Kv4.2, and Kv4.3 mRNAs are present in adult and neonatal rat ventricular myocytes, and that transient outward potassium current (Ito) recovers from inactivation with a slow (Ito,s) and a fast (Ito,f) time course. This study was designed to determine the molecular correlates of Ito,s and Ito,f in cultured neonatal rat ventricular myocytes (NRVM) employing dominant-negative adenoviral infections to manipulate the function of endogenous Ito-encoding K+ channels. Western blot data from cultured NRVM showed that Kv1.4, Kv4.2, and Kv4.3 channel proteins are present in these myocytes. The biphasic recovery from inactivation of Ito in control GFP-infected myocytes demonstrated equal contribution of Ito,s and Ito,f in NRVM. Infection of cultured NRVM with adenoviruses expressing full-length Kv1.4 or Kv4.2 genes generated currents with recovery from inactivation kinetics similar to native Ito,s and Ito,f in GFP-infected myocytes, respectively. Overexpression of dominant-negative truncated Kv1.4 transgene (Kv1.4N) caused a 51% reduction in Ito, selectively removing the slowly recovering Ito,s. Overexpression of dominant-negative Kv4.2N reduced Ito by 53% and eliminated the fast-recovering Ito,f. Our results establish that, in neonatal rat ventricular myocytes, the shaker Kv1 family (probably Kv1.4 and/or Kv1.7) underlies Ito,s, and that the shal Kv4 family (probably Kv4.2 and Kv4.3) is responsible for Ito,f. |
| Starting Page | 351 |
| Ending Page | 358 |
| Page Count | 8 |
| File Format | |
| ISSN | 09462716 |
| Journal | Journal of Molecular Medicine |
| Volume Number | 80 |
| Issue Number | 6 |
| e-ISSN | 14321440 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2002-03-13 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Molecular Medicine Human Genetics Internal Medicine |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Molecular Medicine Genetics (clinical) |
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