NDLI: The effects of inflammatory cytokines on steroidogenic acute regulatory protein expression in macrophages

Content Provider	Springer Nature Link
Author	Ma, Y. Ren, S. Pandak, W. M. Li, X. Ning, Y. Lu, C. Zhao, F. Yin, L.
Copyright Year	2007
Abstract	To investigate the expression of steroidogenic acute regulatory protein (StAR) in macrophages and the effects of inflammatory cytokines on StAR expression.The macrophages isolated from ApoE knockout mice and C57BL/6J mice and RAW264.7 cells (a cell line from mouse macrophage. ATCC Number: TIB-71TM) were cultured in DMEM containing 10% fetal bovine serum. RAW264.7 cells were treated with different inflammatory cytokines (TNF-α, IFN-γ and TGF-β1) and 8-Br-cAMP, a cAMP analog. RT-PCR and Western blot analysis were applied to evaluate the effects of inflammatory cytokines on StAR expression.RT-PCR and Western blot analysis demonstrated the expression of StAR in the macrophages isolated from ApoE knockout mice, C57BL/6J mice and RAW264.7 cells. Proinflammatory cytokines TNF-α and IFN-γ significantly decreased StAR mRNA and protein levels in RAW264.7 cells. The inhibition was dose- and time-dependent. In contrast, anti-inflammatory cytokine TGF-β1 increased StAR mRNA and protein levels. At 1:15 molecular ratio, TGF-β1 blocked the down-regulation of StAR expression mediated by TNF-α. cAMP also induced StAR expression in RAW264.7 cells. When the cells were co-treated with 8-Br-cAMP and TNF-α, 8-Br-cAMP failed to induce StAR expression.Our results provide interesting evidence that inflammatory cytokines regulate StAR expression in macrophages.
Starting Page	495
Ending Page	501
Page Count	7
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	56
Issue Number	12
e-ISSN	1420908X
Language	English
Publisher	SP Birkhäuser Verlag Basel
Publisher Date	2007-12-21
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Steroidogenic acute regulatory protein Tumor necrosis factor-alpha Interferon-gamma Transforming growth factor-beta1 Rheumatology Neurology Dermatology Allergology Pharmacology/Toxicology Immunology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

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