NDLI: Bioelimination of histamine in epithelia of the porcine proximal colon of pigs

Content Provider	Springer Nature Link
Author	Aschenbach, J. R. Honscha, K. U. Vietinghoff, V. Gäbel, G.
Copyright Year	2009
Abstract	The study aimed to gain insight into how intestinal histamine N-methyltransferase (HMT) and diamine oxidase (DAO) could contribute to bioelimination of histamine.Mucosal-to-serosal (ms) and serosal-to-mucosal (sm) fluxes of histamine, choline or 5-hydroxytryptamine were measured in isolated colonic epithelia of pigs (Ussing chambers).Radioactively (hist-rad) and HPLC-measured histamine fluxes were higher in sm vs. ms direction at 100 μM histamine. Choline (3–3000 μM) and 5-HT (20 μM) fluxes only tended to be higher in sm vs. ms direction. Secretion of hist-rad was abolished by serosal 1-methyl-4-phenylpyridinium (MPP). Histamine fluxes accounted for <25 % of hist-rad fluxes, but this percentage increased after blocking HMT (100 μM amodiaquin) or DAO (100 μM aminoguanidine). 1-Methylhistamine (1-MH) appeared exclusively in the serosal medium. 1-MH appearance decreased after addition of amodiaquin or after addition of N-ethylmaleimide (1 mM NEM). Blockage of vesicular trafficking by NEM enhanced histamine catabolism, which could be reversed by aminoguanidine. DAO was detected in punctuate structures in the basal parts of colonocytes by immunohistochemistry.A basolateral organic cation transporter facilitates histamine secretion into the intestinal lumen and delivers histamine to catabolism by HMT and/or vesicular DAO. Histamine is partially released back into the blood after initial biotransformation to 1-MH.
Starting Page	269
Ending Page	276
Page Count	8
File Format	PDF
ISSN	10233830
Journal	Inflammation Research
Volume Number	58
Issue Number	5
e-ISSN	1420908X
Language	English
Publisher	Birkhäuser-Verlag
Publisher Date	2009-01-31
Publisher Place	Basel
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Diamine oxidase Epithelium Histamine Histamine N-methyltransferase Intestine Immunology Pharmacology/Toxicology Rheumatology Allergology Dermatology Neurology
Content Type	Text
Resource Type	Article
Subject	Pharmacology Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Molecular cloning of guinea-pig diamine oxidase and histamine N-methyltransferase

Histamine intolerance: a metabolic disease?

Release and permeation of histamine in isolated caecum epithelia of pigs

Monoclonal antibodies for human and porcine histamine N-methyltransferase (HMT) facilitate protein expression and localization studies

Porcine plasma amine oxidase has a broad substrate specificity and efficiently converts histamine

Single nucleotide polymorphisms and haplotypes of histamine N-methyltransferase in patients with gastric ulcer

Characterisation of functional polymorphisms of the human diamine oxidase gene

Amitriptyline affects guinea-pig post-heparin plasma diamine oxidase activity

The effects of diamine oxidase administration on experimental ulcerative colitis in rats

Bioelimination of histamine in epithelia of the porcine proximal colon of pigs

Similar Documents

Molecular cloning of guinea-pig diamine oxidase and histamine N-methyltransferase

Histamine intolerance: a metabolic disease?

Release and permeation of histamine in isolated caecum epithelia of pigs

Monoclonal antibodies for human and porcine histamine N-methyltransferase (HMT) facilitate protein expression and localization studies

Porcine plasma amine oxidase has a broad substrate specificity and efficiently converts histamine

Single nucleotide polymorphisms and haplotypes of histamine N-methyltransferase in patients with gastric ulcer

Characterisation of functional polymorphisms of the human diamine oxidase gene

Amitriptyline affects guinea-pig post-heparin plasma diamine oxidase activity

The effects of diamine oxidase administration on experimental ulcerative colitis in rats

Bioelimination of histamine in epithelia of the porcine proximal colon of pigs