Loading...
Please wait, while we are loading the content...
Similar Documents
Identification and Sequence Analysis of a Calmodulin-Binding Protein Gene from Citrus Grandis var. Shatinyu Hort
| Content Provider | Semantic Scholar |
|---|---|
| Author | Luo, Qiong Tan, Hongni Huang, Shi-Li Liu, Hua-Ying Qin, Xin-Min |
| Copyright Year | 2017 |
| Abstract | In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus grandis var. Shatinyu Hort were sequenced by high-throughput sequencing technology. A calmodulin-binding protein gene sequence of Citrus grandis var. Shatinyu Hort was obtained though differential analysis method, and some characters of the calmodulin-binding protein were analyzed and predicted by bioinformatics method, including the composition of amino acid sequence, physicochemical parameters, hydrophobicity, transmembrane domain, secondary structure and function of protein etc. The results showed that the calmodulin-binding protein gene was 1616 bp in length with an open reading frame (ORF) of 1368 bp, encoding 455 amino acids with deduced molecular weight of 50.11 KDa, and theoretical pI value of 5.33, and contained a calmodulin-binding proteins domain. Bioinformatics analysis showed that the calmodulin-binding protein was a hydrophobic and unstable protein. The homology analysis of amino acid sequence indicated that the calmodulin-binding protein shared high homology with calmodulin-binding protein of Citrus clementina (98%). This work provides a systemic sequence analysis of the calmodulin-binding protein of Citrus grandis var. Shatinyu Hort. It will provide the useful reference for further investigation of its structure and function. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://pdf.hanspub.org/OJNS20170200000_95058706.pdf |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
