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Identification and Sequence Analysis of β-D-Xylosidase Gene from Citrus grandis var . Shatinyu Hort
| Content Provider | Semantic Scholar |
|---|---|
| Author | Qin, Xinmei Han, Yu Jung Guo, Danni Liu, Yujie Li, Huimin Qin, Xinmin |
| Copyright Year | 2016 |
| Abstract | β-D-Xylosidase gene was identified from the transcriptome of the self-pollinated style and crosspollinated style of Citrus grandis var. Shatinyu Hort. It is 2647 bp (GenBank accession number: KU925841) in length with an open reading frame (ORF) of 2385 bp, encoding 794 amino acids with deduced molecular weight of 87.30 kDa, and theoretical pI value of 5.87. Bioinformatics analysis showed that β-D-Xylosidase is a hydrophilic protein, and contained a same conserved CAS structure domain with the glycosyl hydrolase family. The expression of β-D-Xylosidase gene in unfertilized style was 0.07. in 1 3 day self-pollinated styles were 43.13, 27.84 and 1.95 respectively. Whereas, the expression of β-D-Xylosidase gene in 1 3 day cross-pollinated styles were 33.90, 76.14 and 49.63 respectively. Phylogenetic analysis revealed that β-D-Xylosidase showed closer kinship with that of Citrus sinensis and Citrus clementina, indicating that they belong to the same evolutionary branch. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://www.hanspub.org/journal/PaperDownload.aspx?DOI=10.12677/WJF.2016.54012 |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |