Loading...
Please wait, while we are loading the content...
Similar Documents
Analysis of heparin oligosaccharides by capillary electrophoresis–negative-ion electrospray ionization mass spectrometry
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lin, Lei Liu, Xinyue Zhang, Fuming Chi, Lianli Amster, I. Jonathan Leach, Franklin E. Xia, Qiangwei Linhardt, Robert J. |
| Copyright Year | 2016 |
| Abstract | AbstractMost hyphenated analytical approaches that rely on liquid chromatography–MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization. Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE–MS coupling eletrospray ion source. Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid. The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin. The application of this CE–MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins. Graphical abstractMost hyphenated analytical approaches that rely on liquid chromatography–MS require relatively long separation times, produce incomplete resolution of oligosaccharide mixtures, use eluents that are incompatible with electrospray ionization, or require oligosaccharide derivatization. Here we demonstrate the analysis of heparin oligosaccharides, including disaccharides, ultralow molecular weight heparin, and a low molecular weight heparin, using a novel electrokinetic pump-based CE–MS coupling eletrospray ion source. Reverse polarity CE separation and negative-mode electrospray ionization were optimized using a volatile methanolic ammonium acetate electrolyte and sheath fluid. The online CE hyphenated negative-ion electrospray ionization MS on an LTQ Orbitrap mass spectrometer was useful in disaccharide compositional analysis and bottom-up and top-down analysis of low molecular weight heparin. The application of this CE–MS method to ultralow molecular heparin suggests that a charge state distribution and the low level of sulfate group loss that is achieved make this method useful for online tandem MS analysis of heparins |
| Starting Page | 411 |
| Ending Page | 420 |
| Page Count | 10 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www-heparin.rpi.edu/main/files/papers/5891fd2bd556f9.47876239.pdf |
| PubMed reference number | 27325464v1 |
| Alternate Webpage(s) | https://doi.org/10.1007/s00216-016-9662-1 |
| DOI | 10.1007/s00216-016-9662-1 |
| Journal | Analytical and Bioanalytical Chemistry |
| Volume Number | 409 |
| Issue Number | 2 |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Disaccharides Electrophoresis Electrophoresis, Capillary Heparin, Low-Molecular-Weight Ionization Source Ions Liquid Chromatography Lyase Mass Spectrometers (device) Oligosaccharides Partial Scientific Publication Spectrometry Spectrometry, Mass, Electrospray Ionization ammonium acetate heparin mixture polarity pump (device) |
| Content Type | Text |
| Resource Type | Article |
