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Barbiturates inhibit K+-evoked noradrenaline and dopamine release from rat striatal slices – involvement of voltage sensitive Ca2+ channels
| Content Provider | Semantic Scholar |
|---|---|
| Author | Hirota, Kazuyoshi Kudo, Mihoko Kudo, Tsuyoshi Kitayama, Masato Matsuki, Akitomo |
| Copyright Year | 2000 |
| Abstract | The cellular target site(s) for anaesthetic action remain unclear. In rat striatal slices we have previously demonstrated that K(+)-evoked noradrenaline (NA) and dopamine (DA) release is mediated predominantly via P/Q-type voltage sensitive Ca(2+) channels (VSCC). Using this model of Ca(2+) dependent transmitter release we have evaluated the effects of anaesthetic and non-anaesthetic barbiturates. Rat brain striatal slices were incubated in the absence and presence of barbiturate for 10 min at 37 degrees C. The slices were then incubated for 6 min with 40 mM KCl. All anaesthetic barbiturates produced a concentration-dependent inhibition of K(+)-evoked NA and DA release. Non-anaesthetic barbiturate, barbituric acid was ineffective. The pIC(50) for NA and DA release (thiopental: 4.90+/-0.13 and 5.00+/-0.10, pentobarbital: 4.39+/-0.07 and 4.43+/-0.14, phenobarbital: 3.85+/-0.08 and 3.59+/-0.10, respectively) correlated with lipid solubility (NA: r(2)=0.999, DA: r(2)=0.987). We therefore suggest that barbiturates inhibit catecholamine release via an interaction with P/Q VSCC further implicating this channel in anaesthetic action. |
| Starting Page | 175 |
| Ending Page | 178 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| DOI | 10.1016/S0304-3940(00)01408-7 |
| PubMed reference number | 10984635 |
| Journal | Medline |
| Volume Number | 291 |
| Alternate Webpage(s) | https://api.elsevier.com/content/article/pii/S0304394000014087 |
| Alternate Webpage(s) | https://www.sciencedirect.com/science/article/pii/S0304394000014087?dgcid=api_sd_search-api-endpoint |
| Alternate Webpage(s) | https://doi.org/10.1016/S0304-3940%2800%2901408-7 |
| Journal | Neuroscience Letters |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
