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Curcumin inhibits TGF-β1-induced MMP-9 and invasion through ERK and Smad signaling in breast cancer MDA- MB-231 cells.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Li, Jing Cao, You‐de |
| Copyright Year | 2012 |
| Abstract | OBJECTIVE To evaluate the effects of curcumin on matrixmetalloproteinase-9 (MMP-9) and invasion ability induced by transforming growth factor-β1 (TGF-β1) in MDA-MB-231 cells and potential mechanisms. METHODS Human breast cancer MDA- MB-231 cells were used with the CCK-8 assay to measure the cytotoxicity of curcumin. After treatment with 10 ng/ml TGF-β1, with or without curcumin (≤10 μM), cell invasion was checked by transwell chamber. The effects of curcumin on TGF-β1-stimulated MMP-9 and phosphorylation of Smad2, extracellular-regulated kinase (ERK), and p38 mitogen activated protein kinases (p38MAPK) were examined by Western blotting. Supernatant liquid were collected to analyze the activity of MMP-9 via zymography. Following treatment with PD98059, a specific inhibitor of ERK, and SB203580, a specific inhibitor of p38MAPK, Western blotting and zymography were employed to examine MMP-9 expression and activity, respectively. RESULTS Low dose curcumin (≤10 μM) did not show any obvious toxicity to the cells, while 0~10 μmol/L caused a concentration-dependent reduction in cell invasion provoked by TGF-β1. Curcumin also markedly inhibited TGF-β1-regulated MMP-9 and activation of Smad2, ERK1/2 and p38 in a dose- and time-dependent manner. Additionally, PD98059, but not SB203580, showed a similar pattern of inhibition of MMP-9 expression. CONCLUSION Curcumin inhibited TGF-β1-stimulated MMP-9 and the invasive phenotype in MDA-MB-231 cells, possibly associated with TGF-β/Smad and TGF-β/ERK signaling. |
| Starting Page | 68 |
| Ending Page | 75 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://ocean.kisti.re.kr/downfile/volume/apocp/POCPA9/2012/v13n11/POCPA9_2012_v13n11_5709.pdf |
| PubMed reference number | 23317243v1 |
| Volume Number | 13 |
| Issue Number | 11 |
| Journal | Asian Pacific journal of cancer prevention : APJCP |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | 3,4-Methylenedioxyamphetamine Adverse reaction to drug Cell Invasion Checking (action) Curcumin Leukemia, B-Cell Mammary Neoplasms Mitogen-Activated Protein Kinases Mitogens PD 98059 SB 203580 Sincalide Western Blot Western Blotting mitogen-activated protein kinase p38 |
| Content Type | Text |
| Resource Type | Article |
