Loading...
Please wait, while we are loading the content...
Similar Documents
Detection of astroviruses from stool samples in Japan using reverse transcription and polymerase chain reaction amplification.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Saito, Ken-Ichi Ushijima, Hiroshi Nishio, Osamu Oseto, Mitsuaki Motohiro, Hasegawa Ueda, Yuichi Takagi, Michihiro Nakaya, Shigekazu Ando, Toshio Glass, Roger I. |
| Copyright Year | 1995 |
| Abstract | We developed a reverse transcription and polymerase chain reaction (RT-PCR) method for detecting astrovirus serotypes 1, 2, 3, 5, 6 and 7 (but not serotype 4). Furthermore, we developed the specific primers for detecting serotypes 1 and 2, the most predominant serotypes in the world. Sensitivity of the first PCR with serotype common primers was about 10 times higher than that of enzyme immunoassay with monoclonal antibody (EIA-MAb). Sensitivity of the second PCR with the serotype-specific primers was even higher. The RT-PCR method was useful for detecting astroviruses from clinical samples, especially serotypes 1 and 2. |
| File Format | PDF HTM / HTML |
| DOI | 10.1111/j.1348-0421.1995.tb03264.x |
| Alternate Webpage(s) | https://www.jstage.jst.go.jp/article/mandi1977/39/10/39_10_825/_pdf/-char/en |
| PubMed reference number | 8577275 |
| Alternate Webpage(s) | https://doi.org/10.1111/j.1348-0421.1995.tb03264.x |
| Journal | Medline |
| Volume Number | 39 |
| Issue Number | 10 |
| Journal | Microbiology and immunology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
