NDLI: Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entamoeba dispar DNA from stool samples.

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Multiplex polymerase chain reaction amplification and differentiation of Entamoeba histolytica and Entamoeba dispar DNA from stool samples.

Content Provider	Semantic Scholar
Author	Núñez, Yurai Fernandez, Maria Aparecida Torres-Núñez, David Abner Silva, José Antônio Aleixo Montano, Ivón Maestre, Jorge Luis Fonte, Leyden Martinez
Copyright Year	2001
Abstract	Due to the clinical importance of differentiating the two species of the Entamoeba histolytica/Entamoeba dispar complex, we developed a multiplex polymerase chain reaction (PCR) method that overcomes time-consuming and laborious procedures. We report here a DNA extraction protocol using non-fixed stool samples that avoid long lysis-incubation periods through the combined use of zirconium beads and a lysis-supporting buffer. We characterized 49 of 52 stool specimens from Cuban patients with amoebiosis. Among them, 36 (75.5%) were infected only with E. dispar (the nonpathogenic species), while 13 (24.5%) displayed a mixed infection with both E. dispar and E. histolytica. The multiplex PCR protocol showed a specificity of 1.00 and a sensitivity of 0.94. Furthermore, the entire procedure can be performed in one day. This approach is therefore reliable and applicable in the field for epidemiologic studies.
File Format	PDF HTM / HTML
DOI	10.4269/ajtmh.2001.64.293
PubMed reference number	11463120
Journal	Medline
Volume Number	64
Issue Number	5-6
Alternate Webpage(s)	http://files.sld.cu/digitalizacion-bmn/files/2017/12/T377.05P2.pdf
Alternate Webpage(s)	http://www.ajtmh.org/docserver/fulltext/14761645/64/5/11463120.pdf?accname=guest&checksum=CD209FFBEBE060C382613BBC897D3F35&expires=1546145149&id=id
Alternate Webpage(s)	https://doi.org/10.4269/ajtmh.2001.64.293
Journal	The American journal of tropical medicine and hygiene
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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