NDLI: Nonenzymatic glycation of human lens crystallin. Effect of aging and diabetes mellitus.

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Nonenzymatic glycation of human lens crystallin. Effect of aging and diabetes mellitus.

Content Provider	Semantic Scholar
Author	Garlick, Robert L. Mazer, J. S. Chylack, Leo T. Tung, William H. Bunn, H. Franklin
Copyright Year	1984
Abstract	We have examined the nonenzymatic glycation of human lens crystallin, an extremely long-lived protein, from 16 normal human ocular lenses 0.2-99 yr of age, and from 11 diabetic lenses 52-82-yr-old. The glucitol-lysine (Glc-Lys) content of soluble and insoluble crystallin was determined after reduction with H-borohydride followed by acid hydrolysis, boronic acid affinity chromatography, and high pressure cation exchange chromatography. Normal lens crystallin, soluble and insoluble, had 0.028 +/- 0.011 nanomoles Glc-Lys per nanomole crystallin monomer. Soluble and insoluble crystallins had equivalent levels of glycation. The content of Glc-Lys in normal lens crystallin increased with age in a linear fashion. Thus, the nonenzymatic glycation of nondiabetic lens crystallin may be regarded as a biological clock. The diabetic lens crystallin samples (n = 11) had a higher content of Glc-Lys (0.070 +/- 0.034 nmol/nmol monomer). Over an age range comparable to that of the control samples, the diabetic crystallin samples contained about twice as much Glc-Lys. The Glc-Lys content of the diabetic lens crystallin samples did not increase with lens age.
Starting Page	299
Ending Page	310
Page Count	12
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://dm5migu4zj3pb.cloudfront.net/manuscripts/111000/111592/JCI84111592.pdf
PubMed reference number	6438156v1
Volume Number	74
Issue Number	5
Journal	The Journal of clinical investigation
Language	English
Access Restriction	Open
Subject Keyword	Biological Clocks Borohydrides Cations Chromatography, Affinity Chromatography, Gas-Liquid Contain (action) Crystallins Diabetes Mellitus Diabetic Ketoacidosis Glucose Hyperlysinemias Lysine Mice, Inbred NOD Nanomole Ninety Nine Sorbitol boronic acid monomer
Content Type	Text
Resource Type	Article

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